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Titolo:
Determination of two major metabolites of the novel anti-tumour agent 5,6-dimethylxanthenone-4-acetic acid in hepatic microsomal incubations by high-performance liquid chromatography with fluorescence detection
Autore:
Zhou, SF; Paxton, JW; Tingle, MD; McCall, J; Kestell, P;
Indirizzi:
Univ Auckland, Dept Pharmacol & Clin Pharmacol, Auckland 1, New Zealand Univ Auckland Auckland New Zealand 1 Pharmacol, Auckland 1, New Zealand Univ Auckland, Dept Surg, Auckland 1, New Zealand Univ Auckland AucklandNew Zealand 1 Dept Surg, Auckland 1, New Zealand Univ Auckland, Auckland Canc Soc, Res Ctr, Auckland 1, New Zealand Univ Auckland Auckland New Zealand 1 c, Res Ctr, Auckland 1, New Zealand
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 734, anno: 1999,
pagine: 129 - 136
SICI:
1387-2273(19991029)734:1<129:DOTMMO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; PERFUSED RAT-LIVER; XANTHENONE-4-ACETIC ACID; FLAVONE-8-ACETIC ACID; ANTITUMOR AGENTS; ACYL GLUCURONIDES; COVALENT BINDING; IN-VITRO; ELIMINATION; PHARMACOKINETICS;
Keywords:
5,6-dimethylxanthenone-4-acetic acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Paxton, JW Univ Auckland, Dept Pharmacol & Clin Pharmacol, Auckland 1, NewZealand Univ Auckland Auckland New Zealand 1 Auckland 1, New Zealand
Citazione:
S.F. Zhou et al., "Determination of two major metabolites of the novel anti-tumour agent 5,6-dimethylxanthenone-4-acetic acid in hepatic microsomal incubations by high-performance liquid chromatography with fluorescence detection", J CHROMAT B, 734(1), 1999, pp. 129-136

Abstract

High-performance liquid chromatographic methods have been developed and validated for the glucuronidated and oxidative metabolites of the novel anti-tumour agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA), produced in human liver microsomal incubations. Calibration curves for DMXAA acyl glucuronide (DMXAA-Glu) and 6-hydroxymethyl-5-methylxanthenone-4-acetic acid (6-OH-MXAA) were constructed over the concentration ranges of 0.25 to 20 and 0.5 to 40 mu M, respectively. Assay performance was determined by intra- and inter-day accuracy and precision of quality control (QC) samples. The difference between the theoretical and measured concentration, and the coefficient of variation, were less than 15% at low QC concentrations, and less than 10% at medium and high QC concentrations for both analytes. The methods presented good accuracy, precision and sensitivity for use in kinetic studies ofthe glucuronidated and oxidative metabolites of DMXAA in human liver microsomes. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 10:15:16