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Titolo:
Cloning of the human thiamine transporter, a member of the folate transporter family
Autore:
Dutta, B; Huang, W; Molero, M; Kekuda, R; Leibach, FH; Devoe, LD; Ganapathy, V; Prasad, PD;
Indirizzi:
Med Coll Georgia, Dept Biochem & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 m & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia, Dept Obstet & Gynecol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 et & Gynecol, Augusta, GA 30912 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 45, volume: 274, anno: 1999,
pagine: 31925 - 31929
SICI:
0021-9258(19991105)274:45<31925:COTHTT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC CATION TRANSPORTER; BORDER MEMBRANE-VESICLES; RENAL BRUSH-BORDER; FUNCTIONAL EXPRESSION; HUMAN PLACENTA; INTESTINE; PROTEIN; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Prasad, PD Med Coll Georgia, Dept Biochem & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 l, Augusta, GA 30912 USA
Citazione:
B. Dutta et al., "Cloning of the human thiamine transporter, a member of the folate transporter family", J BIOL CHEM, 274(45), 1999, pp. 31925-31929

Abstract

We have isolated a cDNA from human placenta, which, when expressed heterologously in mammalian cells, mediates the transport of the water-soluble vitamin thiamine. The cDNA codes for a protein of 497 amino acids containing 12 putative transmembrane domains, Northern blot analysis indicates that this transporter is widely expressed in human tissues. When expressed in HeLa cells, the cDNA induces the transport of thiamine (K-i = 2.5 +/- 0.6 mu M) in a Na+-independent manner, The cDNA-mediated transport of thiamine is stimulated by an outwardly directed H+ gradient. Substrate specificity assays indicate that the transporter is specific to thiamine, Even though thiamineis an organic cation, the cDNA-induced thiamine transport is not inhibitedby other organic cations. Similarly, thiamine is not a substrate for the known members of mammalian organic cation transporter family. The thiamine transporter gene, located on human chromosome 1q24, consists of 6 exons and is most likely the gene defective in the metabolic disorder, thiamine-responsive megaloblastic anemia. At the level of amino acid sequence, the thiamine transporter is most closely related to the reduced-folate transporter and thus represents member of the folate transporter family.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:22:12