Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Novel human gene transfer vectors: Evaluation of wild-type and recombinantanimal adenoviruses in human-derived cells
Autore:
Rasmussen, UB; Benchaibi, M; Meyer, V; Schlesinger, Y; Schughart, K;
Indirizzi:
Transgene SA, Dept BMC, F-67082 Strasbourg, France Transgene SA Strasbourg France F-67082 t BMC, F-67082 Strasbourg, France
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 16, volume: 10, anno: 1999,
pagine: 2587 - 2599
SICI:
1043-0342(19991101)10:16<2587:NHGTVE>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERSISTENT TRANSGENE EXPRESSION; IN-VIVO; IMMUNE-RESPONSE; OVINE ADENOVIRUS; NUCLEOTIDE-SEQUENCE; SIGMODON HISPIDUS; INFECTED-CELLS; VIRAL GENES; THERAPY; GENOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Rasmussen, UB Transgene SA, Dept BMC, 11 Rue Molsheim, F-67082 Strasbourg,France Transgene SA 11 Rue Molsheim Strasbourg France F-67082 ance
Citazione:
U.B. Rasmussen et al., "Novel human gene transfer vectors: Evaluation of wild-type and recombinantanimal adenoviruses in human-derived cells", HUM GENE TH, 10(16), 1999, pp. 2587-2599

Abstract

Major disadvantages of human adenovirus (hAd) vectors in gene therapy include preexisting or induced immune responses, and possible coreplication of recombinant hAd in the presence of wild-type hAds. These disadvantages may be overcome by using nonhuman, animal adenoviruses (aAds). We evaluated four different aAds for their potential use as viral vectors. The canine adenovirus type 2 (CAV2) and bovine adenovirus type 3 (BAV3) appeared to be suitable systems, as they infect human cells. CAV2, but not BAV3, caused cytotoxicity, and only limited (CAV2) or no (BAV3) production of infectious virusparticles was observed after infection of human cell lines. CAV2 showed higher expression of endogenous genes than did BAV3 in the tested human cells. No interference between hAd and CAV2 or BAV3, such as recombination of DNA or cross-activation of virus replication, was observed in up to five passages in double-infected human cells. Transfection of cloned genomic CAV2 orBAV3 DNA into appropriate permissive cell lines rescued infectious virus. Furthermore, we produced a recombinant E1-deleted BAV3, and showed that it could infect and express a reporter gene in various human cell types.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:32:52