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Titolo:
Fission yeast mutants that alleviate transcriptional silencing in centromeric flanking repeats and disrupt chromosome segregation
Autore:
Ekwall, K; Cranston, G; Allshire, RC;
Indirizzi:
Western Gen Hosp, Human Genet Unit, MRC, Edinburgh EH4 2XU, Midlothian, Scotland Western Gen Hosp Edinburgh Midlothian Scotland EH4 2XU dlothian, Scotland
Titolo Testata:
GENETICS
fascicolo: 3, volume: 153, anno: 1999,
pagine: 1153 - 1169
SICI:
0016-6731(199911)153:3<1153:FYMTAT>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITION-EFFECT VARIEGATION; MATING-TYPE CASSETTES; SCHIZOSACCHAROMYCES-POMBE; EPIGENETIC INHERITANCE; CHROMATIN STRUCTURE; FUNCTIONAL-ANALYSIS; SPINDLE FORMATION; PROTEIN; MUTATIONS; HETEROCHROMATIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Allshire, RC Western Gen Hosp, Human Genet Unit, MRC, Crewe Rd, Edinburgh EH4 2XU, Midlothian, Scotland Western Gen Hosp Crewe Rd Edinburgh Midlothian Scotland EH4 2XU
Citazione:
K. Ekwall et al., "Fission yeast mutants that alleviate transcriptional silencing in centromeric flanking repeats and disrupt chromosome segregation", GENETICS, 153(3), 1999, pp. 1153-1169

Abstract

In the fission yeast Schizosaccharomyces pombe genes are transcriptionallysilenced when placed within centromeres, within or close to the silent mating-type loci or adjacent to telomeres. Factors required to maintain mating-type silencing also affect centromeric silencing and chromosome segregation. We isolated mutations that alleviate repression of market genes in the inverted repeats flanking the central core of centromere I. Mutations csp1 to 13 (centromere: suppressor of position effect) defined 12 loci. Tell of the csp mutants have no effect on mat2/3 or telomere silencing. All csp mutants allow some expression of genes in the centromeric flanking repeat, but expression in the central core is undetectable. Consistent with defective centromere structure and function, chromosome loss rates are elevated in allcsp mutants. Mutants csp1 to 6 are temperature-sensitive lethal and csp3 and csp6 cells are defective in mitosis at 36 degrees. csp7 to 13 display a high incidence of lagging chromosomes oil late anaphase spindles. Thus, by screening for mutations that disrupt silencing in the flanking region of a fission yeast centromere a novel collection of mutants affecting centromerearchitecture and chromosome segregation has been isolated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:21:47