Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
In-trans regulation of the N-truncated-NIFA protein of Herbaspirillum seropedicae by the N-terminal domain
Autore:
Monteiro, RA; Souza, EM; Yates, MG; Pedrosa, FO; Chubatsu, LS;
Indirizzi:
Univ Fed Parana, Dept Biochem, BR-81531990 Curitiba, Parana, Brazil Univ Fed Parana Curitiba Parana Brazil BR-81531990 BCtiba, Parana, Brazil
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 180, anno: 1999,
pagine: 157 - 161
SICI:
0378-1097(19991115)180:2<157:IROTNP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
KLEBSIELLA-PNEUMONIAE; TRANSCRIPTIONAL ACTIVATOR; ENTERIC BACTERIA; OVER-PRODUCTION; EXPRESSION; GENES; SEQUENCE; BINDING; NTRC;
Keywords:
Herbaspirillum seropedicae; NifA protein; transcription activation; nitrogen fixation; trans-acting inhibition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Chubatsu, LS Univ Fed Parana, Dept Biochem, CP 19046, BR-81531990 Curitiba, Parana, Brazil Univ Fed Parana CP 19046 Curitiba Parana Brazil BR-81531990 BC
Citazione:
R.A. Monteiro et al., "In-trans regulation of the N-truncated-NIFA protein of Herbaspirillum seropedicae by the N-terminal domain", FEMS MICROB, 180(2), 1999, pp. 157-161

Abstract

The NifA protein is responsible for transcription activation of nif genes in the endophytic diazotroph Herbaspirillum seropedicae. When expressed in Escherichia coli this NifA protein is unable to activate the transcription of a Klebsiella pneumoniae nifH::lacZ fusion. However, a form of NifA lacking the N-terminal domain did activate transcription and its activity was not inhibited by ammonium. In this work we show that when expressed separately, the N-terminal domain of H. seropedicae NifA protein can restore ammonium control of the N-truncated NifA activity in E. coli. This effect is dependent on the relative concentrations of the N-terminal domain and the N-truncated protein and suggests that the N-terminal domain behaves in this respect in a manner similar to that of NifL of the gamma proteobacteria. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 18:15:26