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Titolo:
Flow cytometric immunodissection of the human nephron in vivo and in vitro
Autore:
Helbert, MJF; Dauwe, S; De Broe, ME;
Indirizzi:
Univ Antwerp, Dept Nephrol, Antwerp, Belgium Univ Antwerp Antwerp Belgium iv Antwerp, Dept Nephrol, Antwerp, Belgium
Titolo Testata:
EXPERIMENTAL NEPHROLOGY
fascicolo: 5-6, volume: 7, anno: 1999,
pagine: 360 - 376
SICI:
1018-7782(199909/12)7:5-6<360:FCIOTH>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLACENTAL ALKALINE-PHOSPHATASE; PROXIMAL TUBULE CELLS; HUMAN-KIDNEY; COLLECTING DUCT; ALLOGRAFT-REJECTION; PRIMARY CULTURE; IN-VITRO; MEMBRANE; ANTIBODY; TRANSFORMATION;
Keywords:
cell culture methods; tubular epithelial cells; fluorescence-activated cell sorting; isolation and purification; immunodissection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: De Broe, ME Univ Antwerp Hosp, Dept Nephrol & Hypertens, Wilrijkstr 10, B-2650 Edegem,Antwerpen, Belgium Univ Antwerp Hosp Wilrijkstr 10 Edegem Antwerpen Belgium B-2650
Citazione:
M.J.F. Helbert et al., "Flow cytometric immunodissection of the human nephron in vivo and in vitro", EXP NEPHROL, 7(5-6), 1999, pp. 360-376

Abstract

In the present article, we show that flow cytometric immunodissection of cells immediately following their preparation from a tumor nephrectomy specimen is an accurate way of obtaining pure human primary cultures of proximalconvoluted tubule origin, proximal straight tubule origin, distal tubular origin and/or collecting duct origin. By studying the expression of a panelof cell surface markers in these purified cultures, we could identify a number of markers that retain their lineage specificity in vitro. Using theseappropriate stable markers, flow cytometry provides a simple yet accurate way of determining cell composition in previously unsorted (mixed type) tubular epithelial cultures in terms of proximal versus distal tubule/collecting duct subpopulations. Both subpopulations in mixed type cultures are shown to retain functional characteristics of their in vivo counterparts (glucose uptake, hormonal stimulation of adenylate cyclase) as well as cell type-specific response patterns (such as inducibility of cell adhesion and histocompatibility molecules), indicating the usefulness of studying cell responses in vitro in a cell-type-dependent way. Finally we illustrate that multi-parameter flow cytometry is a powerful tool for assessing constitutive characteristics of and/or responses by the distinct cell subpopulations present in mixed type cultures. Copyright (C) 1999 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:03:56