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Titolo:
Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli
Autore:
Yamazoe, M; Onogi, T; Sunako, Y; Niki, H; Yamanaka, K; Ichimura, T; Hiraga, S;
Indirizzi:
Kumamoto Univ, Sch Med, Inst Mol Embryol & Genet, Dept Mol Cell Biol, Kumamoto 8620976, Japan Kumamoto Univ Kumamoto Japan 8620976 Cell Biol, Kumamoto 8620976, Japan
Titolo Testata:
EMBO JOURNAL
fascicolo: 21, volume: 18, anno: 1999,
pagine: 5873 - 5884
SICI:
0261-4189(19991101)18:21<5873:CFOMMA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING; GENE; CALCIUM; CLONING; FTSZ; IDENTIFICATION; CALRETICULIN; MAINTENANCE; REPLICATION; MUTATION;
Keywords:
Ca2+-binding protein; chromosome partitioning; motor protein; MukB-MukE-MukF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Yamazoe, M Kumamoto Univ, Sch Med, Inst Mol Embryol & Genet, Dept Mol CellBiol, Kuhonji 4-24-1, Kumamoto 8620976, Japan Kumamoto Univ Kuhonji 4-24-1Kumamoto Japan 8620976 976, Japan
Citazione:
M. Yamazoe et al., "Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli", EMBO J, 18(21), 1999, pp. 5873-5884

Abstract

mukF, mukE and mukB genes are essential for the process of chromosome partitioning in Escherichia coil, We have studied protein-protein interactions among MukB, MukE and MukF proteins by co-immunoprecipitation and sucrose gradient sedimentation experiments, using mukFEB null cells harboring plasmids carrying the wild-type or mutant-type mukFEB operon, MukB forms a complexwith MukF and MukE, Analysis of mutant MukB proteins suggested that MukF and MukE bind the C-terminal globular domain of MukB, MukF is indispensable for an interaction between MukB and MukE; however, MukF itself is able to associate with MukB even in the absence of MukE. We have also found that MukF has a Ca2+-binding activity. Although purified MukF was able to make a complex either with MukE or MukB, a complex consisting of the three Muk proteins was barely detected in vitro. However, increasing the Ca2+ or Mg2+ concentration in the reaction partially restored complex formation. This suggests that Ca2+ or Mg2+ may be required for the formation of a complex consisting of the three Muk proteins, and thus may participate in a particular step during chromosome partitioning.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:48:47