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Titolo:
Monastral bipolar spindles in meiosis II of male Trichosia pubescens (Sciaridae): Early stages of spindle formation and chromosome orientation
Autore:
Fuge, H;
Indirizzi:
Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany Univ Kaiserslautern Kaiserslautern Germany D-67653 iserslautern, Germany
Titolo Testata:
CELL MOTILITY AND THE CYTOSKELETON
fascicolo: 3, volume: 44, anno: 1999,
pagine: 190 - 201
SICI:
0886-1544(199911)44:3<190:MBSIMI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRANE-FLY SPERMATOCYTES; XENOPUS EGG EXTRACTS; KINESIN-LIKE PROTEIN; KINETOCHORE MICROTUBULES; SELF-ORGANIZATION; DROSOPHILA-MELANOGASTER; ELIMINATION; DIVISION; MORPHOGENESIS; COMPONENTS;
Keywords:
prometaphase; kinetochore orientation; microtubule orientation; chromosome-induced spindle formation; anastral spindle pole;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Fuge, H Univ Kaiserslautern, Dept Biol, POB 3049, D-67653 Kaiserslautern, Germany Univ Kaiserslautern POB 3049 Kaiserslautern Germany D-67653 rmany
Citazione:
H. Fuge, "Monastral bipolar spindles in meiosis II of male Trichosia pubescens (Sciaridae): Early stages of spindle formation and chromosome orientation", CELL MOTIL, 44(3), 1999, pp. 190-201

Abstract

The metaphase spindle of male meiosis II in fungus gnats (Sciaridae) is one example of naturally occurring monastral bipolar spindles. To gain further insights into how the bipolar spindle is formed in the presence of only one polar center, prometaphase of male meiosis II was investigated in the sciarid Trichosia pubescens by means of anti-tubulin immunofluorescence, DAPIchromosome staining, and electron microscopy of ultrathin serial sections. The first step in spindle formation after interkinesis seems to be the organization of an astral half-spindle, probably by MTOC activity of the astral region. With the exception of the non-disjunctional X chromosome, which always lies close to the aster, the chromosomes are found to occupy various positions with respect to the astral region, revealing different orientations of their chromatid kinetochores. It was observed that some of the mal-oriented kinetochores are associated with microtubules that, due to their orientation perpendicular to the spindle axis, are unlikely to originate from the astral region. Therefore, these mal-oriented microtubules are taken as an indication of a dispersed MTOC activity near the chromosomes or at kinetochores. According to recent models of chromosome-induced spindle self-organization [e.g., Merdes et al., 1997. J. Cell Biol. 138:953-956], they couldbe responsible for the formation of the other (anastral) half-spindle and for amphitelic (bipolar) orientation of the chromosomes. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:58:21