Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Enhanced liver uptake of opsonized red blood cells after in vivo transfer of Fc gamma RIIA cDNA to the liver
Autore:
Bezdicek, P; Worgall, S; Kovesdi, I; Kim, MK; Park, JG; Vincent, T; Leopold, PL; Schreiber, AD; Crystal, RG;
Indirizzi:
Cornell Univ, Weill Med Coll, Div Pulm & Crit Care Med, New York, NY USA Cornell Univ New York NY USA Div Pulm & Crit Care Med, New York, NY USA GenVec Inc, Rockville, MD USA GenVec Inc Rockville MD USAGenVec Inc, Rockville, MD USA InKine Pharmaceut Co Inc, Blue Bell, PA USA InKine Pharmaceut Co Inc BlueBell PA USA ceut Co Inc, Blue Bell, PA USA Univ Penn, Sch Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 , Sch Med, Philadelphia, PA 19104 USA
Titolo Testata:
BLOOD
fascicolo: 10, volume: 94, anno: 1999,
pagine: 3448 - 3455
SICI:
0006-4971(19991115)94:10<3448:ELUOOR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOVIRUS-MEDIATED TRANSFER; IMPAIRED FUNCTION; CYSTIC-FIBROSIS; RECEPTORS; CLEARANCE; GENE; INVIVO; CD32; POLYMORPHISM; PHAGOCYTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Crystal, RG Cornell Med Ctr, New York Hosp, Div Pulm & Crit Care Med, 520 E 70th St,ST505, New York, NY 10021 USA Cornell Med Ctr 520 E 70th St,ST 505 New York NY USA 10021 USA
Citazione:
P. Bezdicek et al., "Enhanced liver uptake of opsonized red blood cells after in vivo transfer of Fc gamma RIIA cDNA to the liver", BLOOD, 94(10), 1999, pp. 3448-3455

Abstract

Fc gamma receptors convey to phagocytic cells the ability to recognize, bind, and internalize IgG-coated cells and microorganisms. The present study demonstrates the use of adenovirus (Ad)-mediated gene transfer of human Fc gamma receptor IIA cDNA to convert normally nonphagocytic cells (hepatocytes) into functional equivalents of phagocytic cells. Ad vector in vitro transfer and expression of Fc gamma RIIA cDNA in primary rat hepatocytes was confirmed by flow cytometry anti-Fc gamma RIIA immunodetection, and the function of the receptor was demonstrated by enhanced binding and phagocytosis of Cr-51-labeled IgG-opsonized erythrocytes. After in vivo gene transfer to rats, expression of Fc gamma RIIA cDNA in hepatocytes was confirmed by Northern analysis and immunohistochemistry. Rats infected with the Ad vector carrying the Fc gamma RIIA cDNA demonstrated enhanced clearance of opsonized erythrocytes, but not nonopsonized erythrocytes, from the circulation with increased sequestration within the liver. Together, these data demonstrate that Ad-mediated Fc gamma RIIA gene transfer can convert normally IgG-nonphagocytic cells into phagocytic cells capable of recognizing, binding, and ingesting an opsonized particulate antigen, suggesting that gene transfer strategies might be used to transiently augment host defense by enhancing theclearance of immune complexes. (C) 1999 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 00:38:46