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Titolo:
Ion permeation properties of a cloned human 5-HT3 receptor transiently expressed in HEK 293 cells
Autore:
Mochizuki, S; Miyake, A; Furuichi, K;
Indirizzi:
Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Mol Med Labs, Tsukuba, Ibaraki 305, Japan Yamanouchi Pharmaceut Co Ltd Tsukuba Ibaraki Japan 305 Ibaraki 305, Japan
Titolo Testata:
AMINO ACIDS
fascicolo: 3, volume: 17, anno: 1999,
pagine: 243 - 255
SICI:
0939-4451(1999)17:3<243:IPPOAC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATCH-CLAMP TECHNIQUES; FUNCTIONAL EXPRESSION; PHARMACOLOGICAL CHARACTERIZATION; GANGLION NEURONS; SUBUNIT; CHANNELS; CLONING; SELECTIVITY; RESPONSES; SEROTONIN;
Keywords:
amino acids; serotonin receptor; serotonin-3 receptor; ion channel; ion permeability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Mochizuki, S Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Mol Med Labs, 21 Miyukigaoka, Tsukuba, Ibaraki 305, Japan Yamanouchi Pharmaceut Co Ltd 21 Miyukigaoka Tsukuba Ibaraki Japan 305
Citazione:
S. Mochizuki et al., "Ion permeation properties of a cloned human 5-HT3 receptor transiently expressed in HEK 293 cells", AMINO ACIDS, 17(3), 1999, pp. 243-255

Abstract

Human 5-HT3 receptors expressed in HEK 293 cells were studied using patch-clamp techniques. The permeability ratios of cations to Na+ were Li+, 1.16;K+, 1.04; Rb+, 1.11; Cs+, 1.11; NMDG(+), 0.04; Ca2+, 0.49, and Mg2+, 0.37. The permeability sequence of the alkali metal cations was Li+ > Rb+ = Cs+ > K+ > Na+. Increased external concentrations of Ca2+ or Mg2+ decreased 5-HT-induced currents at all potentials tested in a voltage-independent manner. The single-channel conductance of human 5-HT3 receptors measured by fluctuation analysis of whole-cell currents was 790 +/- 100 fS. Differences in the basic properties of 5-HT3 receptors between species may explain interspecies differences in pharmacological properties.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:39:06