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Titolo:
Motility induced by human immunodeficiency virus-1 Tat on Kaposi's sarcomacells requires platelet-activating factor synthesis
Autore:
Biancone, L; Cantaluppi, V; Boccellino, M; Bussolati, B; Del Sorbo, L; Conaldi, PG; Albini, A; Toniolo, A; Camussi, G;
Indirizzi:
Univ Turin, Dipartimento Med Interna, Cattedra Nefrol, Turin, Italy Univ Turin Turin Italy mento Med Interna, Cattedra Nefrol, Turin, Italy Ist Nazl Ric Canc, I-16132 Genoa, Italy Ist Nazl Ric Canc Genoa Italy I-16132 azl Ric Canc, I-16132 Genoa, Italy Univ Insubria, Dipartimento Sci Clin & Biol, Cattedra Microbiol, Varese, Italy Univ Insubria Varese Italy in & Biol, Cattedra Microbiol, Varese, Italy
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 5, volume: 155, anno: 1999,
pagine: 1731 - 1739
SICI:
0002-9440(199911)155:5<1731:MIBHIV>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-ENDOTHELIAL-CELLS; IN-VIVO ANGIOGENESIS; FACTOR BIOSYNTHESIS; FLK-1/KDR RECEPTOR; ANTAGONIST; PROTEIN; GROWTH; CHEMOTAXIS; MIGRATION; LESIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Camussi, G Osped Maggiore S Giovanni Battista, Dipartimento Med Interna, Cattedra Nefrol, Corso Dogliotti 14, I-10126 Turin, Italy Osped Maggiore S Giovanni Battista Corso Dogliotti 14 Turin Italy I-10126
Citazione:
L. Biancone et al., "Motility induced by human immunodeficiency virus-1 Tat on Kaposi's sarcomacells requires platelet-activating factor synthesis", AM J PATH, 155(5), 1999, pp. 1731-1739

Abstract

In the present study, we evaluated whether motility of Kaposi's sarcoma (KS) spindle cells induced by HIV-1 Tat protein is dependent on the synthesisof platelet-activating factor (PAF). The results obtained indicate that Tat induced a dose-dependent synthesis of PAF from KS cells at a concentration as low as 0.1 ng/ml. PAF production started rapidly after Tat stimulation, peaking at 30 minutes and declining thereafter. Tat-induced cell migration was also a rapid event starting at 30 minutes. The motility was abrogatedby addition of a panel of chemically unrelated PAF receptor antagonists (WEB 2170, CV 3988, CV 6209, and BN 52021), suggesting that the synthesized PAF mediates the motogenic effect of Tat. This effect was also present on cells plated on a type-I collagen-, fibronectin-, or basement membrane extract-coated surface. Expression of PAF receptor-specific mRNA was detected in KS cells. In addition, examination of the cytoskeletal organization showed that Tat-mediated KS cell redistribution of actin filaments and shape change was also inhibited by a PAF receptor antagonist. Moreover, PAF receptor blockade prevented the up-regulation of pi integrin and the down-regulation of alpha v beta 3 observed after stimulation of KS cells with Tat In conclusion, the results of the present study indicate that Tat-induced PAF synthesis plays a critical role in triggering the events involved in motility of KS cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:57:28