Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A transfected cell model for the renal toxin transporter, rOCT2
Autore:
Pan, BF; Sweet, DH; Pritchard, JB; Chen, R; Nelson, JA;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Expt Pediat, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 , Dept Expt Pediat, Houston, TX 77030 USA NIEHS, Lab Pharmacol & Chem, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709 & Chem, Res Triangle Pk, NC 27709 USA
Titolo Testata:
TOXICOLOGICAL SCIENCES
fascicolo: 2, volume: 47, anno: 1999,
pagine: 181 - 186
SICI:
1096-6080(199902)47:2<181:ATCMFT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC CATION TRANSPORTER; FUNCTIONAL-CHARACTERIZATION; ANION TRANSPORTER; RAT-KIDNEY; MOLECULAR-CLONING; P-GLYCOPROTEIN; SECRETION; EXPRESSION; MICE; MECHANISMS;
Keywords:
organic cation transport; cisplatin; daunomycin; vinblastine; 2-chlorodeoxyadenosine; 2 '-deoxytubercidin; tetraethylammonium; rat kidney;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Nelson, JA Univ Texas, MD Anderson Canc Ctr, Dept Expt Pediat, 1515 Holcombe Blvd, Houston, TX 77030 USA Univ Texas 1515 Holcombe Blvd Houston TX USA77030 TX 77030 USA
Citazione:
B.F. Pan et al., "A transfected cell model for the renal toxin transporter, rOCT2", TOXICOL SCI, 47(2), 1999, pp. 181-186

Abstract

A cDNA for the organic cation transporter (rOCT2) of the rat kidney was inserted into the retroviral plasmid pLXSN. This plasmid was used to stably transfect NIH3T3 cells. The transfected cell line exhibited an enhanced rateof tetraethylammonium (TEA) uptake and efflux compared to wild-type NIH3T3cells. Uptake of TEA by the transfected cells was markedly reduced upon incubation at 4 degrees C. When the extracellular pH was lowered from 8.1 to 5.9, uptake was also reduced, suggesting inhibition of rOCT2 by extracellular protons. The apparent K-m for TEA in the transfected cells was 141 mu M. The classical organic cation transport inhibitors, cyanine 863 and cimetidine, produced noncompetitive inhibition with apparent K-i values of 0.81 and 198 mu M, respectively. Daunomycin, vinblastine, and the deoxyadenosine analogs, 2'-deoxytubercidin and 2-chlorodeoxyadenosine, did not appear to besubstrates for rOCT2. However, the anticancer drug, cisplatin, competitively inhibited TEA uptake by rOCT2 with an apparent K-i value of 925 mu M, suggesting that rOCT2 may play a role in its renal secretion. In summary, transfected NIH3T3 cells provide a facile system by which this and other organic ion transporters can be studied.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 00:44:10