Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effective microbial production of poly(4-hydroxybutyrate) homopolymer by Ralstonia eutropha H16
Autore:
Kimura, H; Ohura, T; Takeishi, M; Nakamura, S; Doi, Y;
Indirizzi:
Yamagata Univ, Fac Engn, Dept Mat Sci & Engn, Yamagata 9928150, Japan Yamagata Univ Yamagata Japan 9928150 Sci & Engn, Yamagata 9928150, Japan RIKEN, Inst Phys & Chem Res, Polymer Chem Lab, Wako, Saitama 35101, Japan RIKEN Wako Saitama Japan 35101 lymer Chem Lab, Wako, Saitama 35101, Japan
Titolo Testata:
POLYMER INTERNATIONAL
fascicolo: 11, volume: 48, anno: 1999,
pagine: 1073 - 1079
SICI:
0959-8103(199911)48:11<1073:EMPOPH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-HYDROXYBUTYRATE METABOLISM; ALCALIGENES-EUTROPHUS; POLY(3-HYDROXYBUTYRATE-CO-4-HYDROXYBUTYRATE); COPOLYESTERS; ACIDS; POLYHYDROXYALKANOATES; 3-HYDROXYBUTYRATE; 3-HYDROXYVALERATE; 4-HYDROXYBUTYRATE; BIOSYNTHESIS;
Keywords:
Ralstonia eutropha H16; wild-type strain; propionic acid; ammonium sulfate; poly(rt-hydroxybutyrate) homopolymer; effective microbial production;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Kimura, H Yamagata Univ, Fac Engn, Dept Mat Sci & Engn, Yamagata 9928150, Japan Yamagata Univ Yamagata Japan 9928150 , Yamagata 9928150, Japan
Citazione:
H. Kimura et al., "Effective microbial production of poly(4-hydroxybutyrate) homopolymer by Ralstonia eutropha H16", POLYM INT, 48(11), 1999, pp. 1073-1079

Abstract

The effective microbial production of copolyesters of 3-hydroxybutyrate (3HB) and 4-hydroxybutyrate (4HB) with high mole fractions of 4HB units by a wild-type strain of Ralstonia eutropha H16 was investigated in culture solutions containing 4-hydroxybutyric acid (4HBA) and various carbon substratesin the presence of a nitrogen source such as ammonium sulfate. The addition of glucose or acetic acid to the culture solution containing 4HBA in the presence of ammonium sulfate resulted in the production of random copolymers of P(3HB-co-4HB) with compositions of up to 82mol% 4HB, but the yield of copolymers was less than 7wt% of dried cell weights. In contrast, when n-alkanoic acids such as propionic acid, butyric acid, valeric acid and hexanoic acid, being subject to P-oxidation metabolism in the cell, were used as the co-substrates of 4HBA in the presence of ammonium sulfate, a mixture of copolymers with two different 4HB compositions was produced, and copolyesters with compositions of 93-100mol% 4HB were isolated from chloroform-n-hexane insoluble fractions in the mixture of copolymers. Especially, when this wild-type Ralstonia eutropha H16 was cultivated in a medium containing 4HBA(15 g litre(-1)), propionic acid (15 g litre(-1)) and ammonium sulfate (15g litre(-1)), namely C/N (mol/mol) = 10, the P(4HB) homopolymer was produced at maximally 34wt% of of dry cell weight (7.8 g litre(-1)), and the conversion yield of 4HBA to P(4HB) homopolymer resulted in values as high as 21mol%. (C) 1999 Society of Chemical Industry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:45:26