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Titolo:
High-frequency transformation of oat via microprojectile bombardment of seed-derived highly regenerative cultures
Autore:
Cho, MJ; Jiang, W; Lemaux, PG;
Indirizzi:
Univ Calif Berkeley, Dept Plant & Microbial Sci, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 ial Sci, Berkeley, CA 94720 USA
Titolo Testata:
PLANT SCIENCE
fascicolo: 1, volume: 148, anno: 1999,
pagine: 9 - 17
SICI:
0168-9452(19991015)148:1<9:HTOOVM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEAF BASE SEGMENTS; TRANSGENIC OAT; TISSUE-CULTURES; PLANTS; FERTILE; EXPRESSION; CULTIVARS; L.;
Keywords:
Avena sativa L.; mature seed; embryogenic callus; highly regenerative tissue; transformation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Cho, MJ Univ Calif Berkeley, Dept Plant & Microbial Sci, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 Berkeley, CA 94720 USA
Citazione:
M.J. Cho et al., "High-frequency transformation of oat via microprojectile bombardment of seed-derived highly regenerative cultures", PLANT SCI, 148(1), 1999, pp. 9-17

Abstract

A highly efficient and reproducible transformation system for oat (AL ena sativa L. cv. GAF/Park-1) was developed using microprojectile bombardment of highly regenerative tissues derived from mature seeds. Callus was inducedunder dim light conditions on medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and high cupric sulfate. Highly regenerative tissues, generated from embryogenic callus, were used as a transformation target. From 327 individual explants bombarded with the beta-glucuronidase gene (uidA; gus) and a hygromycin phosphotransferase gene (hpt), 84 independent transgenic events were obtained after an 8-12-week selection period on hygromycin. All events were regenerable, giving an effective transformation frequency of 26%; co-expression of GUS activity occurred in 70% of the independent events. Presence of the foreign genes in DNA from leaf samples of T-0 and T-1 plants was confirmed by polymerase chain reaction (PCR) amplification and:or DNA blot hybridization. Fertility of the plants from the transgenic lines was 63% (24/38) and the transgene(s) was stably transmitted to T-1 and T-2 progeny. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:01:14