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Titolo:
Survey of the Korean population for 9 short tandem repeat loci
Autore:
Um, JY; Kim, EY; Hwang, CH; Oh, SJ; Kim, MY; Lee, KJ; Jang, GY; Chung, YB;
Indirizzi:
ID Gene Inc, Humin Inst Nucle Acid Res, Seoul 138220, South Korea ID Gene Inc Seoul South Korea 138220 Acid Res, Seoul 138220, South Korea Paik Hosp, Inst Mol Biol, Seoul 100032, South Korea Paik Hosp Seoul South Korea 100032 t Mol Biol, Seoul 100032, South Korea Hanhyo Inst Technol, Taejon 305390, South Korea Hanhyo Inst Technol Taejon South Korea 305390 Taejon 305390, South Korea Ewha Womans Univ, Dept Pharmacol, Seoul 120750, South Korea Ewha Womans Univ Seoul South Korea 120750 col, Seoul 120750, South Korea Bionex Inc, Seoul 133791, South Korea Bionex Inc Seoul South Korea 133791 ionex Inc, Seoul 133791, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 5, volume: 9, anno: 1999,
pagine: 526 - 534
SICI:
1016-8478(19991031)9:5<526:SOTKPF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; GENETIC-VARIATION; VARIABLE NUMBER; VNTR LOCI; DNA; AMPLIFICATION; IDENTIFICATION; DISTRIBUTIONS; PATERNITY; HUMTHO1;
Keywords:
capillary PCR; forensic; microsatellite; paternity; population; STR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Chung, YB ID Gene Inc, Humin Inst Nucle Acid Res, Seoul 138220, South Korea ID Gene Inc Seoul South Korea 138220 Seoul 138220, South Korea
Citazione:
J.Y. Um et al., "Survey of the Korean population for 9 short tandem repeat loci", MOL CELLS, 9(5), 1999, pp. 526-534

Abstract

The short tandem repeats with repeat units ranging from two to several nucleotides became a powerful tool in the field of forensic identification andpaternity determination as well as for research in human gene mapping. Allele and genotype frequencies for 9 short tandem repeats including HUMCSF1PO, HUMTH01, HUMPLA2A1, HUMF13A01, HUMCYAR04, HUMLIPOL, HUMHPRTB, HUMCD4, andHUMFABP were determined using PCR and subsequent analysis of the PCR products by denaturing polyacrylamide gel electrophoresis followed by silver-staining. DNA samples were obtained from about 100 Korean people and amplifiedin a thermocycler adopting glass capillaries rather than traditional tubes. We found that the bovine serum albumin was an essential additive for the capillary PCR, presumably to coat the inner surface of the capillary which may adsorb Tag DNA polymerase, The capillary thermocycler was very effective in reducing the cycling time such that most of the amplification reactions could be finished within 30 min albeit the PCR product was less than thatfor the tube systems. All loci except HUMHPRTB met the Hardy-Weinberg expectations according to the exact test. The cumulative power of discrimination (PD) was 0.9999998 and the power of exclusion (POE) for the paternity test was a little low being 0.9873989.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 01:00:56