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Titolo:
Identification of random amplified polymorphic DNA (RAPD) markers highly linked to sex determination in the red alga Gracilaria gracilis
Autore:
Martinez, EA; Destombe, C; Quillet, MC; Valero, M;
Indirizzi:
Univ Sci & Technol Lille 1, CNRS, UPRESA 8016, Lab Genet & Evolut Populat Vegetales, F-59655 Villeneuve Dascq, France Univ Sci & Technol Lille 1 Villeneuve Dascq France F-59655 Dascq, France
Titolo Testata:
MOLECULAR ECOLOGY
fascicolo: 9, volume: 8, anno: 1999,
pagine: 1533 - 1538
SICI:
0962-1083(199909)8:9<1533:IORAPD>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
BULKED SEGREGANT ANALYSIS; LIFE-HISTORY; MITOTIC RECOMBINATION; RHODOPHYTA; PHENOLOGY; POPULATIONS; CHROMOSOME; VERRUCOSA; GENETICS; NUCLEAR;
Keywords:
BSA; Gracilaria gracilis; haplo-diploidy; RAPD; Rhodophyta; sex-linked markers;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Valero, M Univ Sci & Technol Lille 1, CNRS, UPRESA 8016, Lab Genet & Evolut Populat Vegetales, F-59655 Villeneuve Dascq, France Univ Sci & Technol Lille 1 Villeneuve Dascq France F-59655 nce
Citazione:
E.A. Martinez et al., "Identification of random amplified polymorphic DNA (RAPD) markers highly linked to sex determination in the red alga Gracilaria gracilis", MOL ECOL, 8(9), 1999, pp. 1533-1538

Abstract

The random amplified polymorphic DNA (RAPD) technique was employed in the haplo-diploid dioecious species Gracilaria gracilis to identify sex-linked PCR markers. Sixty-nine decamer oligonucleotide primers were tested on two bulks of DNA, one from five haploid males and the other from five haploid females. One of these primers (OPD13) generated a 430-bp fragment specific to males and a 620-bp fragment specific to females. The diploid individuals (tetrasporophytes) showed the co-occurrence of these two fragments. In order to verify the linkage between the sexual phenotypes and these markers, a progeny array of 59 haploid individuals (male and female) born on a diploidindividual was analysed, in all of which the two markers produced by the OPD13 primer segregated perfectly with sex.

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Documento generato il 13/07/20 alle ore 18:08:00