Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cryopreservation of cold-tender apple germplasm
Autore:
Seufferheld, MJ; Stushnoff, C; Forsline, PL; Gonzalez, GHT;
Indirizzi:
Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 ure, Ft Collins, CO 80523 USA Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 tat, Ft Collins, CO 80523 USA USDA, Natl Germplasm Respository Apple & Grape, Plant Genet Resources Unit, Geneva, NY 14456 USA USDA Geneva NY USA 14456 Plant Genet Resources Unit, Geneva, NY 14456 USA
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
fascicolo: 6, volume: 124, anno: 1999,
pagine: 612 - 618
SICI:
0003-1062(199911)124:6<612:COCAG>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENCAPSULATION-DEHYDRATION; SHOOT-TIPS; ACCLIMATION; STRESS; BUDS; TOLERANCE; STABILIZATION; ACCUMULATION; METABOLISM; RAFFINOSE;
Keywords:
alginate encapsulation; dehydration; freeze-desiccation; sucrose; raffinose; Malus sylvestris var. domestica;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Seufferheld, MJ Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 CO 80523 USA
Citazione:
M.J. Seufferheld et al., "Cryopreservation of cold-tender apple germplasm", J AM S HORT, 124(6), 1999, pp. 612-618

Abstract

Unlike cold-hardy apple germplasm, dormant vegetative buds from cold-tender accessions require stabilization of meristematic tissue to protect against injury during desiccation and cryopreservation, Dormant buds of six applecultivars [Malus sylvestris (L. ) Mill. var. domestica (Borkh. ) Mansf. 'Cox's Orange Pippin', 'Einshemer','Golden Delicious', 'Jonagold','K-14', and 'Mutsu'] collected at specific intervals in 1993, 1993, and 1995 at Geneva, N.Y., were stabilized by encapsulation in 5% alginate, treated with step-wise imbibition of 0.5 to 1.0 M sucrose and 0.2 ar raffinose solution, and desiccated with forced air at 0 degrees C. Sugar-alginate stabilization reduced injury during desiccation, increased cold hardiness of the six cold-tender cultivars frozen to -30 degrees C, and improved recovery following cryopreservation of buds collected before optimal cold acclimation was attained. Sucrose tissue levels did not increase following stabilization treatment, but levels of glucose and fructose, and of an unknown disaccharide increased. This procedure used nontoxic cryoprotectants, and has potential to expand the scope of dormant bud cryopreservation to include cold-tender apple germplasm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:56:54