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Titolo:
Macrophage lipoprotein lipase promotes foam cell formation and atherosclerosis in vivo
Autore:
Babaev, VR; Fazio, S; Gleaves, LA; Carter, KJ; Semenkovich, CF; Linton, MF;
Indirizzi:
Vanderbilt Univ, Med Ctr, Dept Med, Nashville, TN 37323 USA Vanderbilt Univ Nashville TN USA 37323 Dept Med, Nashville, TN 37323 USA Vanderbilt Univ, Med Ctr, Dept Pathol, Nashville, TN 37323 USA Vanderbilt Univ Nashville TN USA 37323 pt Pathol, Nashville, TN 37323 USA Vanderbilt Univ, Med Ctr, Dept Pharmacol, Nashville, TN 37323 USA Vanderbilt Univ Nashville TN USA 37323 Pharmacol, Nashville, TN 37323 USA Washington Univ, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 ington Univ, St Louis, MO 63110 USA
Titolo Testata:
JOURNAL OF CLINICAL INVESTIGATION
fascicolo: 12, volume: 103, anno: 1999,
pagine: 1697 - 1705
SICI:
0021-9738(199906)103:12<1697:MLLPFC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; DIET-INDUCED ATHEROSCLEROSIS; TRANSGENIC MICE; APOLIPOPROTEIN-E; PREMATURE ATHEROSCLEROSIS; KNOCKOUT MICE; FETAL LIVER; STEM-CELLS; RECEPTOR; HYPERTRIGLYCERIDEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Linton, MF Vanderbilt Univ, Sch Med, Div Endocrinol, 715 Med Res Bldg II, Nashville, TN 37232 USA Vanderbilt Univ 715 Med Res Bldg II Nashville TN USA 37232 USA
Citazione:
V.R. Babaev et al., "Macrophage lipoprotein lipase promotes foam cell formation and atherosclerosis in vivo", J CLIN INV, 103(12), 1999, pp. 1697-1705

Abstract

Expression of lipoprotein lipase (LPL) by the macrophage has been proposedto promote foam cell formation and atherosclerosis, primarily on the basisof in vitro studies. LPL-deficient mice might provide a model for testing the role of LPL secretion by the macrophage in an in vivo system. Unfortunately, homozygous deficiency of LPL in the mouse is lethal shortly after birth. Because the fetal liver is the major site of hematopoiesis in the developing fetus, transplantation of C57BL/6 mice with LPL-/- fetal liver cells (FLCs) was used to investigate the physiologic role of macrophage LPL expression in vivo. Thirty-four female C57BL/6 mice were lethally irradiated andreconstituted with FLCs from day 14 LPL+/+, LPL+/-, and LPL-/- donors. No significant differences were detected in plasma levels of post-heparin LPL activity or in serum cholesterol or triglyceride levels between the 3 groups on either a chow diet or an atherogenic diet. After 19 weeks on the atherogenic diet, aortae were collected for quantitative analysis of the extent of aortic atherosclerosis. LPL expression was detected by immunocytochemistry and in situ hybridization in macrophages of aortic atherosclerotic lesions of LPL+/+-->C57BL/6 and LPL+/--->C57BL/6 mice, but not in LPL-/--->C57BL/6 mice, whereas myocardial cells expressed LPL in all groups. The mean aortic lesion area was reduced by 55% in LPL-/--->C57BL/6 mice compared with LPL+/+-->C57BL/6 mice and by 45% compared with LPL+/--->C57BL/6 mice, respectively. These data demonstrate in vivo that LPL expression by macrophages in the artery wall promotes foam cell formation and atherosclerosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:31:07