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Titolo:
Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions
Autore:
Wan, H; Winton, HL; Soeller, C; Tovey, ER; Gruenert, DC; Thompson, PJ; Stewart, GA; Taylor, GW; Garrod, DR; Cannell, MB; Robinson, C;
Indirizzi:
St George Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, London SW17 0RE,England St George Hosp London England SW17 0RE harmacol, London SW17 0RE,England Univ Sydney, Dept Med, Inst Resp Med, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 sp Med, Sydney, NSW 2006, Australia Univ Calif San Francisco, Cardiovasc Res Inst, Dept Lab Med, Gene Therapy Core Ctr, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Western Australia, Dept Med, Nedlands, WA 6907, Australia Univ Western Australia Nedlands WA Australia 6907 nds, WA 6907, Australia Univ Western Australia, Dept Microbiol, Nedlands, WA 6907, Australia Univ Western Australia Nedlands WA Australia 6907 nds, WA 6907, Australia Univ London Imperial Coll Sci Technol & Med, Sch Med, Clin Pharmacol Sect,London W12 0NN, England Univ London Imperial Coll Sci Technol & Med London England W12 0NN gland Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England
Titolo Testata:
JOURNAL OF CLINICAL INVESTIGATION
fascicolo: 1, volume: 104, anno: 1999,
pagine: 123 - 133
SICI:
0021-9738(199907)104:1<123:DP1FTA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOUSE-DUST MITE; DERMATOPHAGOIDES-PTERONYSSINUS; POSSIBLE INVOLVEMENT; MONOCLONAL-ANTIBODY; MEMBRANE-PROTEINS; CHILDHOOD ASTHMA; RISK FACTOR; OCCLUDIN; CELLS; PERMEABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, C St George Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, Cranmer Terrace,London SW17 0RE, England St George Hosp Cranmer Terrace LondonEngland SW17 0RE ngland
Citazione:
H. Wan et al., "Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions", J CLIN INV, 104(1), 1999, pp. 123-133

Abstract

House dust mite (HDM) allergens are important factors in the increasing prevalence of asthma. The lung epithelium forms a barrier that allergens mustcross before they can cause sensitization. However, the mechanisms involved are unknown. Here we show that the cysteine proteinase allergen Der p 1 from fecal pellets of the HDM Dermatophagoides pteronyssinus causes disruption of intercellular tight junctions (TJs), which are the principal components of the epithelial paracellular permeability barrier. In confluent airwayepithelial cells, Der p 1 led to cleavage of the TJ adhesion protein occludin. Cleavage was attenuated by antipain, but not by inhibitors of serine, aspartic, or matrix metalloproteinases. Putative Der p 1 cleavage sites were found in peptides from an extracellular domain of occludin and in the TJ adhesion protein claudin-1. TJ breakdown nonspecifically increased epithelial permeability, allowing Der p 1 to cross the epithelial barrier. Thus, transepithelial movement of Der p 1 to dendritic antigen-presenting cells viathe paracellular pathway may be promoted by the allergen's own proteolyticactivity. These results suggest that opening of TJs by environmental proteinases may be the initial step in the development of asthma to a variety ofallergens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:25:33