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Titolo:
Use of selective antagonists and antisense oligonucleotides to evaluate the mechanisms of BUBU antinociception
Autore:
Hutcheson, DM; Sanchez-Blazquez, P; Rodriguez-Diaz, M; Garzon, J; Schmidhammer, H; Borsodi, A; Roques, BP; Maldonado, R;
Indirizzi:
UFR Sci Pharmaceut & Biol, CNRS UMR 8600, INSERM U266, Dept Pharmacochim Mol & Struct, F-75270 Paris, France UFR Sci Pharmaceut & Biol Paris FranceF-75270 ct, F-75270 Paris, France CSIC, Inst Neurobiol Santiago Ramon Y Cajal, Madrid 28002, Spain CSIC Madrid Spain 28002 biol Santiago Ramon Y Cajal, Madrid 28002, Spain Innsbruck Univ, Inst Pharmaceut Chem, A-6020 Innsbruck, Austria Innsbruck Univ Innsbruck Austria A-6020 Chem, A-6020 Innsbruck, Austria Hungarian Acad Sci, Biol Res Ctr, Inst Biochem, H-6701 Szeged, Hungary Hungarian Acad Sci Szeged Hungary H-6701 Biochem, H-6701 Szeged, Hungary Hungarian Acad Sci, Biol Res Ctr, Isotope Lab, H-6701 Szeged, Hungary Hungarian Acad Sci Szeged Hungary H-6701 ope Lab, H-6701 Szeged, Hungary Albert Szent Gyorgyi Med Univ, Dept Pharmacodynam, H-6721 Szeged, Hungary Albert Szent Gyorgyi Med Univ Szeged Hungary H-6721 6721 Szeged, Hungary
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 1, volume: 383, anno: 1999,
pagine: 29 - 37
SICI:
0014-2999(19991021)383:1<29:UOSAAA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELTA-OPIOID RECEPTOR; ENDOGENOUS ENKEPHALINS; SPINAL ANTINOCICEPTION; INDUCED ANALGESIA; BIOLOGICAL EVALUATION; BINDING-SITES; HIGH-AFFINITY; MU-RECEPTORS; IN-VIVO; MICE;
Keywords:
BUBU; delta-opioid receptor; cyprodime; naltrindole; antisense oligonucleotide; antinociception;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Maldonado, R Univ Pompeu Fabra, Fac Ciencies Salut Vida, Lab Neurofarmacol, C Dr Aiguader 80, Barcelona 08003, Spain Univ Pompeu Fabra C Dr Aiguader 80 Barcelona Spain 08003 ain
Citazione:
D.M. Hutcheson et al., "Use of selective antagonists and antisense oligonucleotides to evaluate the mechanisms of BUBU antinociception", EUR J PHARM, 383(1), 1999, pp. 29-37

Abstract

Evidence suggests that the antinociceptive effects of selective delta-opioid receptor agonists may involve an activation of the mu-receptor in some experimental conditions. The aim of this study was to clarify the receptors involved in the antinociceptive responses of the selective and systemicallyactive delta-opioid receptor agonist Tyr-D-Ser-(O-tert-butyl)-Gly-Phe-Leu-Thr-(O-tert-butyl) (BUBU). The antinociception induced by systemic (i.v.) or central (i.c.v.) administration of BUBU was measured in the hot plate (jumping and paw lick latencies) and tail immersion tests in mice. In both tests, the responses were more intense when BUBU was administered by central route. The pre-treatment with the mu-opioid receptor antagonist cyprodime blocked the effects induced by central BUBU in the hot plate and tail immersion tests. The delta-opioid receptor antagonist naltrindole had no effect onBUBU-induced antinociception in the hot plate but decreased BUBU responsesin the tail immersion test. Further evidence for this dual receptor actionof BUBU was demonstrated by using antisense oligodeoxynucleotides. Thus, areduction in central BUBU-induced antinociception was observed in the tailimmersion test after the administration of antisense probes that selectively blocked the expression of mu- or delta-opioid receptors. These findings clearly indicate using a dual pharmacological and molecular approach that BUBU mediates its antinociceptive effects via activation of both mu- and delta-opioid receptors. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:40:27