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Titolo:
Energy metabolism following prolonged hepatic cold preservation: Benefits of interrupted hypoxia on the adenine nucleotide pool in rat liver
Autore:
Mitchell, SJ; Churchill, TA; Winslet, MC; Fuller, BJ;
Indirizzi:
Univ Coll London, Royal Free Hosp, Sch Med, Dept Surg,Liver Transplant Unit, London NW3 2QG, England Univ Coll London London England NW3 2QG nt Unit, London NW3 2QG, England Univ Alberta, Surg Med Res Inst, Edmonton, AB T6G 2N8, Canada Univ Alberta Edmonton AB Canada T6G 2N8 nst, Edmonton, AB T6G 2N8, Canada
Titolo Testata:
CRYOBIOLOGY
fascicolo: 2, volume: 39, anno: 1999,
pagine: 130 - 137
SICI:
0011-2240(199909)39:2<130:EMFPHC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIAL RESPIRATORY-FUNCTION; ISCHEMIC PRESERVATION; MACHINE PERFUSION; UW-SOLUTION; P-31 NMR; TRANSPLANTATION; HYPOTHERMIA; REPERFUSION; MAINTENANCE; PURINE;
Keywords:
liver preservation; hypothermic reperfusion; interrupted hypoxia; energy metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Fuller, BJ Univ Coll London, Royal Free Hosp, Sch Med, Dept Surg,Liver Transplant Unit, Pond St, London NW3 2QG, England Univ Coll London Pond St London England NW3 2QG 3 2QG, England
Citazione:
S.J. Mitchell et al., "Energy metabolism following prolonged hepatic cold preservation: Benefits of interrupted hypoxia on the adenine nucleotide pool in rat liver", CRYOBIOLOGY, 39(2), 1999, pp. 130-137

Abstract

The ability of brief hypothermic reperfusion (HtR) to restore hepatic energy metabolism following periods of cold hypoxic preservation was studied inisolated rat livers after storage times of 5, 10, and 24 h. In addition, investigations were performed on the effects of HtR used to restore Liver oxidative metabolism in the middle of a prolonged (24 h) hypoxic preservationperiod. A histidine-lactobionate-raffinose solution was used for the initial cold portal flush in all groups. Results showed that cold hypoxia for either 5 or 10 h yielded livers capable of similar recoveries of ATP, energy charge, and total adenine nucleotides, but that HtR after 24 h cold preservation resulted in reduced regeneration of ATP, a lower energy charge, and afall in tissue adenine nucleotides. When Livers were stead for 24 h but subjected to brief HtR after either 5 or 10 h before return to hypoxic storage, improved recoveries of the energy metabolites were seen over those recorded after 24 h hypoxia alone. The fact that these improvements were not dueto an improved supply of adenine nucleotide precursors was demonstrated bystudying groups which were given HtR with perfusate containing precursors of adenine nucleotides (adenosine, adenine, and inosine) after 24 h cold hypoxia. These data are consistent with the hypothesis that poor metabolic recovery after long-term hepatic cold preservation results more from decreased mitochondrial oxidative phosphorylation than from a lack of precursors for adenine nucleotide resynthesis. In addition, restoring oxidative metabolism at hypothermia for brief periods can to some extent protect final metabolic status after prolonged storage. (C) 1999 Academic Press.

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Documento generato il 19/09/20 alle ore 15:19:58