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Titolo:
Genomic nucleotide sequence of a foot-and-mouth disease virus clone and its persistent derivatives - Implications for the evolution of viral quasispecies during a persistent infection
Autore:
Toja, M; Escarmis, C; Domingo, E;
Indirizzi:
Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, CSIC, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 a, CSIC, E-28049 Madrid, Spain
Titolo Testata:
VIRUS RESEARCH
fascicolo: 2, volume: 64, anno: 1999,
pagine: 161 - 171
SICI:
0168-1702(199911)64:2<161:GNSOAF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CULTURE; ANTIGENIC VARIANTS; 5'-UNTRANSLATED REGION; IMMUNE SELECTION; RNA VIRUSES; B VIRUS; HETEROGENEITY; DIVERSIFICATION; APHTHOVIRUS; MUTATIONS;
Keywords:
persistent infection; coevolution; mutation frequency; viral phenotype;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Domingo, E Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, CSIC, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 28049 Madrid, Spain
Citazione:
M. Toja et al., "Genomic nucleotide sequence of a foot-and-mouth disease virus clone and its persistent derivatives - Implications for the evolution of viral quasispecies during a persistent infection", VIRUS RES, 64(2), 1999, pp. 161-171

Abstract

The consensus nucleotide sequence of the entire genome of foot-and-mouth disease virus (FMDV) (biological clone C-S8c1) has been completed, and compared with that of two persistent derivatives R99 and R146, rescued after 99 and 146 passages of the carrier BHK-21 cells. Consensus sequences were determined directly from supernatants of persistently infected cells, without intervening cytolytic amplification of the viruses. These genomic sequences have also been compared with that of FMDV R100, a virus that was also rescued from persistently infected cells, but that was subjected to cytolytic amplification prior to sequencing. Mutation frequencies for R99 and R146 relative to C-S8c1 were in the range of 2.8 x 10(-3) to 7.7 x 10(-3) substitutions per nucleotide for the 5'-UTR and the L-, P1-, P2- and P3-coding regions. No mutations were fixed in the polymerase (3D)-coding region. Striking contrasts were noted regarding the distribution of mutation types along the persistent genomes, notably the complete absence of transversion mutations within the 5'-UTR, compared with 53% transversions in the L- and pi-coding regions. The sequencing results presented here, combined with previous sequences of FMDV C-S8c1 genomes at the onset of persistence, provide evidence of sequence fluctuations with a non-linear accumulation of mutations duringprolonged persistence, a hallmark of quasispecies dynamics. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 04/12/20 alle ore 06:55:06