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Titolo:
Humoral and cellular immune responses to HIV-1 Nef in mice DNA-immunised with non-replicating or self-replicating expression vectors
Autore:
Collings, A; Pitkanen, J; Strengell, M; Tahtinen, M; Pitkanen, J; Lagerstedt, A; Hakkarainen, K; Ovod, V; Sutter, G; Ustav, M; Ustav, E; Mannik, A; Ranki, A; Peterson, P; Krohn, K;
Indirizzi:
Univ Tampere, Inst Med Technol, FIN-33101 Tampere, Finland Univ Tampere Tampere Finland FIN-33101 chnol, FIN-33101 Tampere, Finland GSF, Natl Ctr Environm & Hlth Res, Inst Mol Virol, Munich, Germany GSF Munich Germany Environm & Hlth Res, Inst Mol Virol, Munich, Germany Estonian Bioctr, Dept Microbiol & Virol, Tartu, Estonia Estonian Bioctr Tartu Estonia r, Dept Microbiol & Virol, Tartu, Estonia Tartu State Univ, EE-202400 Tartu, Estonia Tartu State Univ Tartu Estonia EE-202400 Univ, EE-202400 Tartu, Estonia Univ Helsinki, Dept Dermatol & Venerol, Helsinki, Finland Univ Helsinki Helsinki Finland pt Dermatol & Venerol, Helsinki, Finland Tampere Univ Hosp, Tampere, Finland Tampere Univ Hosp Tampere FinlandTampere Univ Hosp, Tampere, Finland
Titolo Testata:
VACCINE
fascicolo: 5-6, volume: 18, anno: 1999,
pagine: 460 - 467
SICI:
0264-410X(19991014)18:5-6<460:HACIRT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; CYTOTOXIC T-LYMPHOCYTES; ANTIGEN PRESENTATION; DENDRITIC CELLS; GENE-TRANSFER; IN-VITRO; IMMUNIZATION; INFECTION; VACCINES; HOST;
Keywords:
HIV-1 Nef; DNA immunisation; antibodies; cytotoxic T-lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Krohn, K Univ Tampere, Inst Med Technol, FIN-33101 Tampere, Finland Univ Tampere Tampere Finland FIN-33101 N-33101 Tampere, Finland
Citazione:
A. Collings et al., "Humoral and cellular immune responses to HIV-1 Nef in mice DNA-immunised with non-replicating or self-replicating expression vectors", VACCINE, 18(5-6), 1999, pp. 460-467

Abstract

Objective: HIV accessory protein Nef is expressed early in the infectious cycle of the virus and has been shown to be an effective immunogen in humoral and cellular immune responses. We have used two different self-replicating pBN vectors and one non-replicating pCGal2 derived (pCG) vector expressing HIV-1 Nef in DNA immunisation of mice in order to determine their efficiency in raising humoral and cellular immune responses. Design and methods: The expression of Nef by the three plasmids was tested by transfections into COS-1 cells. Balb/c mice were immunised with the pBN-NEF and pCGE2-NEF constructs using gold particle bombardment. Immunoblotting and immunocytochemistry were used to detect in vitro expression of Nef. Cr-51 release assay, ELISA and immunoblotting were used to detect cellular and humoral immune responses in immunised mice. Results: Efficient in vitro expression of Nef was detected in pBN and pCGE2-NEF transfected cells, in pBN-NEF transfected cells the expression lasting up to three weeks. Anti-Nef antibodies in sera of 13 of 16 pBN-NEF immunised mice were detected within four weeks after the last immunisation, whereas only 2 of 12 pCGE2-NEF immunised mice had veryweak anti-Nef antibodies. Twelve of the pBN-NEF immunised mice (75%) and 6the pCGE2-NEF immunised mice (50%) showed Nef-specific cytotoxic T lymphocyte (CTL) responses within four weeks. Conclusions: We conclude that the three eukaryotic expression vectors tested are capable of inducing a cell mediated immune response towards HIV-1 Nef and should be considered as part ofa genetic HIV vaccine. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 17:42:24