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Titolo:
Intermolecular forces between acetylcholine and acetylcholinesterases studied with atomic force microscopy
Autore:
Zhang, YG; Bai, CL; Wang, C; Zhao, DL; Su, M; Lin, Z; Tian, F;
Indirizzi:
Acad Mil Med Sci, Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China Acad Mil Med Sci Beijing Peoples R China 100850 100850, Peoples R China Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China Chinese Acad Sci Beijing Peoples R China 100080 100080, Peoples R China
Titolo Testata:
SCIENCE IN CHINA SERIES B-CHEMISTRY
fascicolo: 5, volume: 42, anno: 1999,
pagine: 449 - 457
SICI:
1001-652X(199910)42:5<449:IFBAAA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TORPEDO-CALIFORNICA;
Keywords:
atomic force microscopy; acetylcholinesterases; acetylcholine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, YG Acad Mil Med Sci, Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China Acad Mil Med Sci Beijing Peoples R China 100850 eoples R China
Citazione:
Y.G. Zhang et al., "Intermolecular forces between acetylcholine and acetylcholinesterases studied with atomic force microscopy", SCI CHINA B, 42(5), 1999, pp. 449-457

Abstract

With the aid of atomic force microscopy, the intermolecular forces betweenacetylcholinesterases (AChE) and its natural substrate acetylcholine (ACh)have been studied. Through force spectrum measurement based on imaging of AChE molecules it was found that the attraction force between individual molecule pairs of ACh and AChE was (10 +/- 1) pN just before the quaternary ammonium head of ACh got into contact with the negative end of AChE and the decaying distance of attraction was (4 +/- 1) nm from the surface of AChE. The adhesion force between individual ACh and AChE molecule pairs was (25 +/- 2) pN, which had a decaying feature of fast-slow-fast (FSF). The attraction forces between AChE and choline (Ch), the quaternary ammonium moiety and hydrolysate of ACh molecule, were similar to those between AChE and ACh. The adhesion forces between AChE and Ch were (20 +/- 2) pN, a little weakerthan that between ACh and AChE. These results indicated that AChE had a steering role for the diffusion of ACh toward it and had resistance against the leave of Ch along the original way along which it came into contact withAChE as the quaternary moiety of ACh.

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Documento generato il 01/12/20 alle ore 22:49:17