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Titolo:
Photodynamic therapy: Tumor targeting with adenoviral proteins
Autore:
Allen, CM; Sharman, WM; La Madeleine, C; Weber, JM; Langlois, R; Ouellet, R; van Lier, JE;
Indirizzi:
Univ Sherbrooke, Fac Med, Dept Med Nucl & Radiobiol, Sherbrooke, PQ J1H 5N4, Canada Univ Sherbrooke Sherbrooke PQ Canada J1H 5N4 erbrooke, PQ J1H 5N4, Canada Univ Sherbrooke, Fac Med, Dept Microbiol, Sherbrooke, PQ J1H 5N4, Canada Univ Sherbrooke Sherbrooke PQ Canada J1H 5N4 erbrooke, PQ J1H 5N4, Canada
Titolo Testata:
PHOTOCHEMISTRY AND PHOTOBIOLOGY
fascicolo: 4, volume: 70, anno: 1999,
pagine: 512 - 523
SICI:
0031-8655(199910)70:4<512:PTTTWA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; CHINESE-HAMSTER CELLS; CHLOROALUMINUM PHTHALOCYANINE TETRASULFONATE; SINGLET OXYGEN GENERATION; MEDIATED GENE-TRANSFER; ALPHA-V INTEGRINS; HUMAN LUNG-CANCER; BIOLOGICAL-ACTIVITIES; ZINC(II)-PHTHALOCYANINE-CONTAINING LIPOSOMES; SULFONATED PHTHALOCYANINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
95
Recensione:
Indirizzi per estratti:
Indirizzo: van Lier, JE Univ Sherbrooke, Fac Med, Dept Med Nucl & Radiobiol, Sherbrooke, PQ J1H 5N4, Canada Univ Sherbrooke Sherbrooke PQ Canada J1H 5N4 J1H 5N4, Canada
Citazione:
C.M. Allen et al., "Photodynamic therapy: Tumor targeting with adenoviral proteins", PHOTOCHEM P, 70(4), 1999, pp. 512-523

Abstract

A brief summary of the mechanisms involved in photodynamic therapy (PDT) and the role of delivery vehicles for photosensitizer targeting is addressed. Phthalocyanines (Pc) have been coupled to adenovirus type 2 capsid proteins including the hexon, the penton base and the fiber to enhance their target selectivity. Adenovirus penton base proteins contain the arginine-glycine-aspartic acid peptidic sequence (RGD) moth known to bind with great affinity and high specificity to integrin receptors, expressed by several types of cancer. Tetrasulfonated aluminum phthalocyanine (AIPcS(4)) was covalently coupled tee the various capsid proteins via one or two caproic acid spacer chains (A(1) of A(2)) in 7:1 up to 66:1 molar ratios, The capacity of thebioconjugates for singlet oxygen production, as measured by an L-tryptophan oxidation assay, was strongly reduced, likely reflecting scavenging by the carrier. Cell adsorption and in vitro photocytotoxicity assays were carried out using the A549 and HEp2 human cell lines expressing integrin receptors, and one murine, the EMT-6 cell line, which lacks receptors for the RGD sequence, The AIPcS(4)A(2)-protein complexes induced greater cytotoxicity as compared to the analogous AIPcS(4)A(1) preparations. The penton base-AIPcS(4)A(2) derivative was the more phototoxic for all cell lines tested. Tumor response studies using Balb/c mice with EMT-6 tumor implants demonstratedthat the free AIPcS(4)A(2), induced complete tumor regression at a dose of1 mu mol/ kg and 400 J/cm(2), which is comparable to the activity of the known AIPcS2(adj). A mixture of adenovirus type 2 soluble proteins covalently labeled with AIPcS(4)A(2) required 0.5 mu mol/kg to induce the same response with the same light dose, suggesting that the high affinity RGD/receptor complex is able to target Pc for PDT.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:43:13