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Titolo:
[H-3] gamma-aminobutyric acid transport in rat substantia nigra pars reticulata synaptosomes: pharmacological characterization and phorbol ester-induced inhibition
Autore:
Bahena-Trujillo, R; Arias-Montano, JA;
Indirizzi:
Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Fisiol, Mexico City 07000, DF, Mexico Inst Politecn Nacl Mexico City DF Mexico 07000 ico City 07000, DF, Mexico
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 2, volume: 274, anno: 1999,
pagine: 119 - 122
SICI:
0304-3940(19991022)274:2<119:[GATIR>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; BRAIN GABA TRANSPORTER; XENOPUS-OOCYTES; MOUSE-BRAIN; EXPRESSION; GAT1; LOCALIZATION; ACTIVATION; RECEPTORS;
Keywords:
GAT-1; gamma-aminobutyric acid; gamma-aminobutyric acid uptake; substantia nigra; basal ganglia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Bahena-Trujillo, R Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Fisiol, Apdo Postal 14-740, Mexico City 07000, DF, Mexico Inst Politecn Nacl Apdo Postal 14-740 Mexico City DF Mexico 07000
Citazione:
R. Bahena-Trujillo e J.A. Arias-Montano, "[H-3] gamma-aminobutyric acid transport in rat substantia nigra pars reticulata synaptosomes: pharmacological characterization and phorbol ester-induced inhibition", NEUROSCI L, 274(2), 1999, pp. 119-122

Abstract

In synaptosomes from rat substantia nigra pars reticulata, [H-3] gamma-aminobutyric acid (GABA) uptake was inhibited by GABA, (+/-)-nipecotic acid, beta-alanine and SKF 89976-A. Inhibition was concentration-dependent and monophasic, with IC50 values that agree with those reported for the cloned ratGABA transporter GAT-1. [H-3]GABA uptake was modestly, but significantly, reduced (21 +/- 3% inhibition) by 100 nM phorbol 12-tetradecanoyl-13-acetate (TPA), an activator of protein kinase C (PKC). The inhibitory action of TPA was reversed by the PKC inhibitor staurosporine (100 nM). Saturation analysis revealed that TPA reduced the maximum capacity of transport with no change in the affinity for GABA. [3H]GABA uptake was unaffected by either forskolin (10 mu M) or 8-bromo-cAMP (500 mu M). These results indicate that SNr GABAergic afferents express the GAT-1 transporter whose activity can be regulated by a PKC-mediated mechanism. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:16:00