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Titolo:
The intracellular domain of the nicotinic acetylcholine receptor alpha subunit mediates its coclustering with rapsyn
Autore:
Maimone, MM; Enigk, RE;
Indirizzi:
SUNY Hlth Sci Ctr, Dept Anat & Cell Biol, Syracuse, NY 13210 USA SUNY HlthSci Ctr Syracuse NY USA 13210 Cell Biol, Syracuse, NY 13210 USA
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 4-5, volume: 14, anno: 1999,
pagine: 340 - 354
SICI:
1044-7431(199910/11)14:4-5<340:TIDOTN>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTSYNAPTIC 43-KDA PROTEIN; NEUROMUSCULAR-JUNCTIONS; BETA-SUBUNIT; MUSCLE-CELLS; ION CHANNEL; CLUSTERS; TORPEDO; MEMBRANES; ASSOCIATION; MUTAGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Maimone, MM SUNY Hlth Sci Ctr, Dept Anat & Cell Biol, Syracuse, NY 13210 USA SUNY Hlth Sci Ctr Syracuse NY USA 13210 yracuse, NY 13210 USA
Citazione:
M.M. Maimone e R.E. Enigk, "The intracellular domain of the nicotinic acetylcholine receptor alpha subunit mediates its coclustering with rapsyn", MOL CELL NE, 14(4-5), 1999, pp. 340-354

Abstract

Muscle nicotinic acetylcholine receptors (AChRs) are immobilized at the neuromuscular junction in high-density clusters by rapsyn, a 43-kDa protein located at the cytoplasmic face of the postsynaptic membrane. When expressedin nonmuscle cells, rapsyn induces the aggregation of both assembled and unassembled AChR subunits. Here, we investigated the mechanism of rapsyn-induced clustering of the AChR alpha subunit by testing a series of or subunitmutants for colocalization with rapsyn patches in transfected QT6 cells. Partial or total deletion of the large intracellular domain of the alpha subunit dramatically reduced its ability to colocalize with rapsyn patches. Furthermore, insertion of the alpha subunit Targe intracellular domain into apotassium channel resulted in a significant increase in the channel's colocalization with rapsyn patches. We conclude that the large intracellular domain of the alpha subunit plays an important role in mediating rapsyn-induced co-clustering of the AChR alpha subunit.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:28:53