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Titolo:
Age-related alteration of intracellular ATP maintenance in the cell suspensions of mice cerebral cortex
Autore:
Joo, HJ; Ma, JY; Choo, YG; Choi, BK; Jung, KY;
Indirizzi:
Wonkwang Univ, Coll Nat Sci, Sch Med, Dept Cellular & Mol Pharmacol, Chonbuk 570749, South Korea Wonkwang Univ Chonbuk South Korea 570749 ol, Chonbuk 570749, South Korea Wonkwang Univ, Coll Nat Sci, Dept Mol Embryol, Chonbuk 570749, South KoreaWonkwang Univ Chonbuk South Korea 570749 ol, Chonbuk 570749, South Korea
Titolo Testata:
MECHANISMS OF AGEING AND DEVELOPMENT
fascicolo: 1-2, volume: 110, anno: 1999,
pagine: 1 - 12
SICI:
0047-6374(19991001)110:1-2<1:AAOIAM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-TRANSPORT CHAIN; RAT-BRAIN; ALZHEIMERS-DISEASE; ENERGY-METABOLISM; GLUCOSE-METABOLISM; BLOOD-FLOW; DAMAGE; SURVIVAL; NEURONS;
Keywords:
age; cell suspension; cerebral cortex; intracellular ATP content; in vitro; mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Jung, KY Wonkwang Univ, Coll Nat Sci, Sch Med, Dept Cellular & Mol Pharmacol, 344-2Shinyong-dong, Chonbuk 570749, South Korea Wonkwang Univ 344-2 Shinyong-dong Chonbuk South Korea 570749 rea
Citazione:
H.J. Joo et al., "Age-related alteration of intracellular ATP maintenance in the cell suspensions of mice cerebral cortex", MECH AGE D, 110(1-2), 1999, pp. 1-12

Abstract

Neurological alteration in the aging brain has been suggested to be due to, in part, a declined cellular energy metabolism. In order to understand age-related alteration of intracellular ATP maintenance, the present in vitrostudy measured change of intracellular adenosine triphosphate (ATP) content in cell suspensions of cerebral cortex isolated from male ICR mice aged 2days (infant), 8 weeks (young adult) and 12 months (aged) under several different conditions, using the chemiluminescence technique. Among the three different ages, significant decrease of intracellular ATP content by oxygendeprivation for 15 min was observed in the cell suspensions of cerebral cortex from la-month-old mice (P < 0.05). When cell suspensions of 8-week cerebral cortex were incubated with or without glucose (0-60 min), intracellular ATP content decreased in a time-dependent manner under both conditions, but depletion rate was significantly high in the glucose-free condition. This decrease was maximally restored by adding 1 mM glucose as tested. In addition, the ability for intracellular ATP maintenance in the presence or absence of glucose was age-dependently different. The rank order of differenceof intracellular ATP content between with and without glucose was 3 months> 12 months > 2 days. The highest decrease of intracellular ATP content byincubation without glucose was observed in the 12-month samples. Sodium cyanide (100 mu M) produced a gradual ATP depletion in cerebral cortex suspended from 2-day-old mice, but rapid change in both 8-week and 12-month samples. Combination of cyanide and iodoacetate (3.5 mM) rapidly depleted the intracellular ATP content in all age groups tested. These results suggest that the aging process in the cerebral cortex of mice is accompanied by alteration of maintenance of intracellular ATP homeostasis under a given condition, and this may be associated with pathological change of overall mechanisms involved in the development of neuronal disease in the senescent brain. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 14:54:42