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Titolo:
Identification of clusterin sequences mediating renal tubular cell interactions
Autore:
Silkensen, JR; Skubitz, APN; Skubitz, KM; Rosenberg, ME;
Indirizzi:
Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 pt Med, Minneapolis, MN 55455 USA Univ Florida, Dept Med, Gainesville, FL USA Univ Florida Gainesville FL USA v Florida, Dept Med, Gainesville, FL USA Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 Pathol, Minneapolis, MN 55455 USA Univ Minnesota, Ctr Biomed Engn, Minneapolis, MN 55455 USA Univ MinnesotaMinneapolis MN USA 55455 d Engn, Minneapolis, MN 55455 USA
Titolo Testata:
JOURNAL OF PEPTIDE RESEARCH
fascicolo: 5, volume: 54, anno: 1999,
pagine: 449 - 457
SICI:
1397-002X(199911)54:5<449:IOCSMR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; SULFATED GLYCOPROTEIN-2; MESSENGER-RNA; APOLIPOPROTEIN-J; PROTEIN CLUSTERIN; MOLECULAR-CLONING; TESTIS FLUID; IN-VITRO; COMPLEMENT; DEATH;
Keywords:
adhesion; aggregation; apolipoprotein; apolipoprotein J; clusterin; injury;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Rosenberg, ME Univ Minnesota, Dept Med, Box 736 UMHC,420 Delaware St SE, Minneapolis, MN55455 USA Univ Minnesota Box 736 UMHC,420 Delaware St SE Minneapolis MN USA 55455
Citazione:
J.R. Silkensen et al., "Identification of clusterin sequences mediating renal tubular cell interactions", J PEPT RES, 54(5), 1999, pp. 449-457

Abstract

Expression of the glycoprotein clusterin is markedly increased following tissue injury. One function of clusterin is to promote cell interactions which are perturbed in these pathologic settings. Clusterin causes cell aggregation and adhesion in vitro yet the molecular mechanism for this effect is not known. In order to identify the active site(s) of clusterin, 34 peptides, each 15 amino acid residues in length, were synthesized from hydrophilicregions of human clusterin. When studied individually, none of the peptides caused aggregation of LLC-PK1 cells, a porcine renal epithelial cell line. However, two out of the 34 peptides inhibited clusterin-induced cell aggregation in a dose-dependent manner. Scrambled versions of these two 'active' peptides did not inhibit cell aggregation. Seven peptides promoted cell adhesion. In conclusion, these findings provide evidence for novel amino acid sequences mediating clusterin-induced renal cell interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:38:24