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Titolo:
Identification of a cis-acting dendritic targeting element in MAP2 mRNAs
Autore:
Blichenberg, A; Schwanke, B; Rehbein, M; Garner, CC; Richter, D; Kindler, S;
Indirizzi:
Univ Hamburg, Inst Cell Biochem & Clin Neurobiol, D-20246 Hamburg, GermanyUniv Hamburg Hamburg Germany D-20246 Neurobiol, D-20246 Hamburg, Germany Univ Alabama, Dept Neurobiol, S Birmingham, AL 35213 USA Univ Alabama S Birmingham AL USA 35213 robiol, S Birmingham, AL 35213 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 20, volume: 19, anno: 1999,
pagine: 8818 - 8829
SICI:
0270-6474(19991015)19:20<8818:IOACDT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCAL PROTEIN-SYNTHESIS; MESSENGER-RNA LOCALIZATION; IMMEDIATE-EARLY GENE; HIPPOCAMPAL-NEURONS; MOLECULAR CHARACTERIZATION; POSTSYNAPTIC SITES; BC1 RNA; CULTURE; TRANSPORT; EXPRESSION;
Keywords:
dendritic targeting element; RNA localization into dendrites; subcellular transport; extrasomatic protein synthesis; neuronal cytoskeleton/microtubule-associated protein; primary cultures of neurons; microinjection; transfection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Kindler, S Univ Hamburg, Inst Cell Biochem & Clin Neurobiol, Martinistr 52, D-20246 Hamburg, Germany Univ Hamburg Martinistr 52 Hamburg Germany D-20246 rg, Germany
Citazione:
A. Blichenberg et al., "Identification of a cis-acting dendritic targeting element in MAP2 mRNAs", J NEUROSC, 19(20), 1999, pp. 8818-8829

Abstract

In neurons, a limited number of mRNAs have been identified in dendritic processes, whereas other transcripts are restricted to the cell soma. Here wehave investigated the molecular mechanisms underlying extrasomatic localization of mRNAs encoding microtubule-associated protein 2 (MAP2) in primary neuronal cultures. Vectors expressing recombinant mRNAs were introduced into hippocampal and sympathetic neurons using DNA transfection and microinjection protocols, respectively. Chimeric mRNAs containing the entire 3' untranslated region of MAP2 transcripts fused to a nondendritic reporter mRNA are detected in dendrites. In contrast, RNAs containing MAP2 coding and 5' untranslated regions or tubulin sequences are restricted to the cell soma. Moreover, 640 nucleotides from the MAP2 3' untranslated region (UTR) are bothsufficient and essential for extrasomatic localization of chimeric mRNAs in hippocampal and sympathetic neurons. Thus, a cis-acting dendritic targeting element that is effective in two distinct neuronal cell types is contained in the 3' UTR of MAP2 transcripts. The observation of RNA granules in dendrites implies that extrasomatic transcripts seem to assemble into multimolecular complexes that may function as transport units.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:45:00