Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Loss of postsynaptic GABA(A) receptor clustering in gephyrin-deficient mice
Autore:
Kneussel, M; Brandstatter, JH; Laube, B; Stahl, S; Muller, U; Betz, H;
Indirizzi:
Max Planck Inst Brain Res, Dept Neurochem, D-60528 Frankfurt, Germany Max Planck Inst Brain Res Frankfurt Germany D-60528 8 Frankfurt, Germany Max Planck Inst Brain Res, Dept Neuroanat, D-60528 Frankfurt, Germany Max Planck Inst Brain Res Frankfurt Germany D-60528 8 Frankfurt, Germany
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 21, volume: 19, anno: 1999,
pagine: 9289 - 9297
SICI:
0270-6474(19991101)19:21<9289:LOPGRC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT SPINAL-CORD; MICROTUBULE-ASSOCIATED PROTEIN-5; GAMMA-AMINOBUTYRIC ACID; TUBULIN LINKER PROTEIN; GLYCINE-RECEPTOR; GLUTAMATERGIC SYNAPSES; LOCALIZATION; EXPRESSION; SUBUNITS; NEURONS;
Keywords:
GABA(A) receptor; gephyrin; receptor clustering; knock-out mice; hippocampal cultures; NMDA receptor; AMPA receptor; PSD-95;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Betz, H Max Planck Inst Brain Res, Dept Neurochem, Deutschordenstr 46, D-60528 Frankfurt, Germany Max Planck Inst Brain Res Deutschordenstr 46 Frankfurt Germany D-60528
Citazione:
M. Kneussel et al., "Loss of postsynaptic GABA(A) receptor clustering in gephyrin-deficient mice", J NEUROSC, 19(21), 1999, pp. 9289-9297

Abstract

The tubulin-binding protein gephyrin, which anchors the inhibitory glycinereceptor (GlyR) at postsynaptic sites, decorates GABAergic postsynaptic membranes in various brain regions, and postsynaptic gephyrin clusters are absent from cortical cultures of mice deficient for the GABA(A) receptor gamma 2 subunit. Here, we investigated the postsynaptic clustering of GABA(A) receptors in gephyrin knock-out (geph -/-) mice. Both in brain sections and cultured hippocampal neurons derived from geph -/- mice, synaptic GABA(A) receptor clusters containing either the gamma 2 or the alpha 2 subunit were absent, whereas glutamate receptor subunits were normally localized at postsynaptic sites. Western blot analysis and electrophysiological recording revealed that normal levels of functional GABA(A) receptors are expressed in geph -/- neurons, however the pool size of intracellular GABA(A) receptors appeared increased in the mutant cells. Thus, gephyrin is required for the synaptic localization of GlyRs and GABA(A) receptors containing the gamma 2and/or alpha 2 subunits but not for the targeting of these receptors to the neuronal plasma membrane. In addition, gephyrin may be important for efficient membrane insertion and/or metabolic stabilization of inhibitory receptors at developing postsynaptic sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 06:11:25