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Titolo:
THE ROLE OF MYOEPITHELIAL-DERIVED GROWTH-FACTORS IN THE HUMAN BREAST
Autore:
COOMBES RC; BULUWELA L; GOMM JJ;
Indirizzi:
CHARING CROSS & WESTMINSTER MED SCH,DEPT MED ONCOL,CANC RES CAMPAIGN LONDON W6 8RP ENGLAND CHARING CROSS & WESTMINSTER MED SCH,DEPT BIOCHEM LONDON W6 8RP ENGLAND
Titolo Testata:
Endocrine-related cancer
fascicolo: 1, volume: 4, anno: 1997,
pagine: 35 - 43
SICI:
1351-0088(1997)4:1<35:TROMGI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMARY EPITHELIAL-CELLS; FACTOR-BETA; CARCINOMA-CELLS; FACTOR FAMILY; ACTIVIN-A; EXPRESSION; GLAND; DIFFERENTIATION; SPECIFICITY; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
R.C. Coombes et al., "THE ROLE OF MYOEPITHELIAL-DERIVED GROWTH-FACTORS IN THE HUMAN BREAST", Endocrine-related cancer, 4(1), 1997, pp. 35-43

Abstract

We have studied separated human breast epithelial and myoepithelial cells for the presence of basic fibroblast growth factor (FGF2) and itsreceptors and for the effects of FGF2 on the proliferation of both cell types. We have also studied the role of activin and its receptor incontrolling cell proliferation. Our results indicated that these celltypes differ markedly in synthesis and response to FGF2 and activin and in their receptor content. FGF2 had no effect on the proliferation of myoepithelial cells but promoted the survival of the separated epithelial cells. Immunostainable FGF receptors 1 and 4 were present in epithelial cells and to a lesser extent in myoepithelial cells. These results indicated that myoepithelial-derived FGF2 may be important in controlling epithelial cell survival and that differential receptor expression could control FGF2 action in these different cell types. We found that activin beta-a and activin type II receptor are expressed by myoepithelial cells, whereas no expression was detected in other breastcell types. In examining 15 breast cell lines, we found only four (HBL-100, MCF-10A, PMC-42 and BT-20) to be positive for activin beta-a mRNA, whereas all expressed the activin type II receptor. Furthermore, we have found activin A to be a potent growth inhibitor of MCF-7 cells where it causes an arrest in G(1). Activin A does not appear to have an effect on the cell cycle of primary myoepithelial or luminal cells. However, we have demonstrated that activin is an inhibitor of tubule formation by human mammary organoids in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 09:48:28