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Titolo:
Dichotomy of cytokine profiles in patients and high-risk healthy subjects exposed to tuberculosis
Autore:
Bhattacharyya, S; Singla, R; Dey, AB; Prasad, HK;
Indirizzi:
All India Inst Med Sci, Dept Biotechnol, New Delhi 110030, India All IndiaInst Med Sci New Delhi India 110030 l, New Delhi 110030, India All India Inst Med Sci, Dept Med, New Delhi 110030, India All India Inst Med Sci New Delhi India 110030 d, New Delhi 110030, India LRS Inst TB & Allied Dis, New Delhi 110030, India LRS Inst TB & Allied Dis New Delhi India 110030 New Delhi 110030, India
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 11, volume: 67, anno: 1999,
pagine: 5597 - 5603
SICI:
0019-9567(199911)67:11<5597:DOCPIP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIDRUG-RESISTANT TUBERCULOSIS; MYCOBACTERIUM-TUBERCULOSIS; INTERFERON-GAMMA; T-CELLS; PULMONARY TUBERCULOSIS; HUMAN-MONOCYTES; LYMPHOKINE PRODUCTION; IMMUNE-RESPONSE; CALMETTE-GUERIN; IFN-GAMMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Prasad, HK All India Inst Med Sci, Dept Biotechnol, New Delhi 110029, India All India Inst Med Sci New Delhi India 110029 i 110029, India
Citazione:
S. Bhattacharyya et al., "Dichotomy of cytokine profiles in patients and high-risk healthy subjects exposed to tuberculosis", INFEC IMMUN, 67(11), 1999, pp. 5597-5603

Abstract

To better understand the role of cytokines in susceptible and resistant subjects exposed to Mycobacterium tuberculosis infection, intracellular gammainterferon (IFN-gamma) and interleukin-4 (IL-4) in ex vivo peripheral blood-derived CD4(+) T cells were examined by flow cytometry, Of the 37 individuals examined, 20 had clinical evidence of pulmonary tuberculosis and showed acid-fast bacilli in the sputum, Other individuals in close contact with these patients showed no evidence of disease. Patients had a higher number of CD4(+) T cells expressing IFN-gamma and IL-4 in unstimulated cultures compared to healthy subjects. Despite this, the ratio of IFN-gamma(+) to IL-4(+) CD4(+) T cells was similar in both groups, The Th1 response seen in CD4(+) T cells in patients was also observed in the overall pattern of IFN-gamma and IL-4 detected in control culture supernatants by enzyme-linked immunosorbent assay (ELISA), However, after in vitro stimulation of PBMC with heat-killed M, tuberculosis there was a significant reduction in the percentage of IFN-gamma(+) CD4(+) T cells (P < 0.001) in patients. This trend was reflected in the IFN-gamma ELISA assay with supernatants derived from stimulated cultures. However, the accumulated levels of IFN-gamma were higher than those for IL-4. The reduction of IFN-gamma(+) CD4(+) T cells resulted in the dominance of IL-4(+) CD4(+) T cells in 13 patients (P < 0.05), The elevated levels of IL-4(+) CD4(+) T cells seen in patients may contribute to the downregulation of IFN-gamma expression and the crucial effector function of CD4 T cells, leading to the persistence of disease and the immunopathology characteristically seen in patients. Preliminary data on the indicators of apoptosis in antigen-stimulated cultures in PBMC derived from patients are presented. Of the 17 high-risk healthy individuals examined, 11 differedin that, after mycobacterial-antigen stimulation, there was an enhancementin IFN-gamma(+) CD4(+) T cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:11:13