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Titolo:
Expression of cholecystokinin A receptors in neurons innervating the rat stomach and intestine
Autore:
Sternini, C; Wong, H; Pham, T; de Giorgio, R; Miller, LJ; Kuntz, SM; Reeve, JR; Walsh, JH; Raybould, HE;
Indirizzi:
W Los Angeles Vet Affairs Med Ctr, CURE Digest Dis Res Ctr, Los Angeles, CA 90073 USA W Los Angeles Vet Affairs Med Ctr Los Angeles CA USA 90073 , CA 90073 USA Univ Calif Los Angeles, Dept Med, Div Digest Dis, CURE Digest Dis Res Ctr,Los Angeles, CA 90024 USA Univ Calif Los Angeles Los Angeles CA USA 90024 Los Angeles, CA 90024 USA Univ Calif Los Angeles, Dept Neurobiol, Div Digest Dis, CURE Digest Dis Res Ctr, Los Angeles, CA 90024 USA Univ Calif Los Angeles Los Angeles CA USA90024 Los Angeles, CA 90024 USA Univ Calif Los Angeles, Dept Physiol, Div Digest Dis, CURE Digest Dis Res Ctr, Los Angeles, CA 90024 USA Univ Calif Los Angeles Los Angeles CA USA 90024 Los Angeles, CA 90024 USA Univ Calif Los Angeles, Inst Brain Res, Los Angeles, CA 90024 USA Univ Calif Los Angeles Los Angeles CA USA 90024 Los Angeles, CA 90024 USA Univ Bologna, Dept Internal Med & Gastroenterol, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 Gastroenterol, I-40126 Bologna, Italy Mayo Clin & Mayo Fdn, Ctr Basic Res & Digest Dis, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Dis, Rochester, MN 55905 USA
Titolo Testata:
GASTROENTEROLOGY
fascicolo: 5, volume: 117, anno: 1999,
pagine: 1136 - 1146
SICI:
0016-5085(199911)117:5<1136:EOCARI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBTYPE-SPECIFIC ANTIBODIES; MESSENGER-RNA EXPRESSION; GASTRIC-ACID SECRETION; GASTROINTESTINAL-TRACT; PEPTIDE IMMUNOREACTIVITY; FUNCTIONAL EXPRESSION; VAGAL INNERVATION; AFFERENT NEURONS; DIGESTIVE-SYSTEM; SUBSTANCE-P;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Sternini, C W Los Angeles Vet Affairs Med Ctr, CURE Digest Dis Res Ctr, 11301 WilshireBlvd,Bldg 115,Room 2245, Los Angeles, CA 90073 USA W Los Angeles Vet Affairs Med Ctr 11301 Wilshire Blvd,Bldg 115,Room 2245 Los Angeles CAUSA 90073
Citazione:
C. Sternini et al., "Expression of cholecystokinin A receptors in neurons innervating the rat stomach and intestine", GASTROENTY, 117(5), 1999, pp. 1136-1146

Abstract

Background & Aims: Two distinct receptors, cholecystokinin (CCK)-A and CCK-B, mediate CCK effects in the digestive system. The aim of this study was to elucidate the cellular sites of expression of CCK-A receptor in the rat stomach and small intestine. Methods: We developed and characterized an antibody to the N-terminal region (LDQPQPSKEWQSA) of rat CCK-A receptor and used it for localization studies with immunohistochemistry. Results: Specificity of the antiserum was demonstrated by (1) detection of a broad band at 85-95 kilodaltons in Western blots of membranes from CCK-A receptor CHO-transfected cells; (2) cell surface staining of CCK-A receptor-transfected cells, (3) translocation of CCK-A receptor immunostaining in CCK-A receptor-transfected cells after exposure to CCK; and (4) abolition of tissue immunostaining by preadsorbtion of the antibody with the peptide used for immunization. CCK-A receptor immunoreactivity was localized to myenteric neurons and to fibers in the muscle and mucosa. In the stomach, myenteric neurons and mucosal fibers were abundant. Many CCK-A receptor myenteric neurons contained the inhibitory transmitter vasoactive intestinal polypeptide, and some were immunoreactive for the excitatory transmitter substance P. Subdiaphragmatic vagotomy reduced the density of CCK-A receptor fibers in the gastric mucosa by approximately 50%, whereas celiac/superior mesenteric ganglionectomy had no detectable effect on fiber density. Conclusions: CCK-A receptor isexpressed in functionally distinct neurons of the gastrointestinal tract. CCK-A receptor may mediate reflexes stimulated by CCK through the release of other transmitters from neurons bearing the receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:06:37