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Titolo:
Potentiation of glutamatergic agonist-induced inositol phosphate formationby basic fibroblast growth factor is related to developmental features in hippocampal cultures: neuronal survival and glial cell proliferation
Autore:
Blanc, EM; Jallageas, M; Recasens, M; Guiramand, J;
Indirizzi:
Univ Montpellier 2, CNRS, EP628, Lab Plast Cerebrale, F-34095 Montpellier 5, France Univ Montpellier 2 Montpellier France 5 e, F-34095 Montpellier 5, France
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 10, volume: 11, anno: 1999,
pagine: 3377 - 3386
SICI:
0953-816X(199910)11:10<3377:POGAIP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY AMINO-ACIDS; LONG-TERM POTENTIATION; STIMULATED PHOSPHOINOSITIDE TURNOVER; MGLUR1 MUTANT MICE; PHOSPHOLIPID HYDROLYSIS; SYNAPTIC PLASTICITY; NEURITE EXTENSION; RECEPTOR SUBUNIT; FACTOR PROMOTES; NERVOUS-SYSTEM;
Keywords:
AMPA receptors; in vitro development; metabotropic glutamate receptors; rat hippocampal cultures; trophic factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Guiramand, J Univ Montpellier 2, CNRS, EP628, Lab Plast Cerebrale, CC 090,F-34095 Montpellier 5, France Univ Montpellier 2 CC 090 Montpellier France 5 ier 5, France
Citazione:
E.M. Blanc et al., "Potentiation of glutamatergic agonist-induced inositol phosphate formationby basic fibroblast growth factor is related to developmental features in hippocampal cultures: neuronal survival and glial cell proliferation", EUR J NEURO, 11(10), 1999, pp. 3377-3386

Abstract

We investigated the modulation by growth factors of phospholipase C (PLC)-linked glutamate receptors during in vitro development of hippocampal cultures. In defined medium, glial cells represent between 3 and 14% of total cell number. When we added basic fibroblast growth factor (bFGF) 2 h after plating, we found: (i) a neuroprotection from naturally occurring death for up to 5 days; (ii) a proliferation of glial cells from day 3; and (iii) a potentiation of quisqualate (QA)-induced inositol phosphate (IP) formation from 1 to 10 days in vitro (DIV) and 1S,3R-amino-cyclopentane-1,3-dicarboxylate (ACPD) response from 3 to 10 DIV. The antimitotic cytosine-beta,D-arabinofuranoside (AraC) blocked glial cell proliferation induced by bFGF, but not neuroprotection. Under these conditions, the early potentiation of the QAresponse (1-3 DIV) was not changed, while the ACPD and late QA response potentiations were prevented (5-10 DIV). Epidermal growth factor was not neuroprotective but it induced both glial cell proliferation and late QA or ACPD potentiation. Surprisingly, the early bFGF-potentiated QA-induced IP response was blocked by 6,7-dinitro-quinoxaline-2,3-dione (DNQX), suggesting the participation of ionotropic (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)/kainate (KA) receptors. The delayed bFGF-potentiated ACPD-induced IP response is inhibited by (S)-alpha-methyl-4-carboxyphenylglycine (MCPG), indicating possible activation of glial metabotropic receptors. These results suggest that, in hippocampal cultures, bFGF modulates AMPA and metabotropic glutamate receptors linked to the IP cascade, possibly in relation to the regulation of neuronal survival and glial cell proliferation, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 13:28:41