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Titolo:
A kinetic model for the homing and migration of prenatally transplanted marrow
Autore:
Shaaban, AF; Kim, HB; Milner, R; Flake, AW;
Indirizzi:
Childrens Hosp Philadelphia, Ctr Fetal Diag & Treatment, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA
Titolo Testata:
BLOOD
fascicolo: 9, volume: 94, anno: 1999,
pagine: 3251 - 3257
SICI:
0006-4971(19991101)94:9<3251:AKMFTH>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; IN-UTERO TRANSPLANTATION; SEVERE COMBINED IMMUNODEFICIENCY; BONE-MARROW; MOLECULAR-BASIS; MOUSE FETUSES; FETAL LIVER; MICE; VIVO; ENDOTHELIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Flake, AW Childrens Hosp Philadelphia, Ctr Fetal Diag & Treatment, 34th St& Civic Ctr Blvd, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia 34th St & Civic Ctr Blvd Philadelphia PA USA 19104
Citazione:
A.F. Shaaban et al., "A kinetic model for the homing and migration of prenatally transplanted marrow", BLOOD, 94(9), 1999, pp. 3251-3257

Abstract

Currently little is known about the mechanisms regulating the homing and the early engraftment of prenatally transplanted hematopoietic cells due to the lack of a relevant functional assay. In this study, we have defined a reproducible kinetic profile of the homing and the early engraftment events in a murine model of prenatal stem cell transplantation. Light density mononuclear cells (LDMCs) from adult C57Pep3b and SJL/J marrow were transplanted by intraperitoneal (IP) injection into C57BL/6 fetuses (10(6) LDMCs/fetus) at 14 days of gestation. The fetuses were sacrificed at early time points(1.5 to 96 hours) after transplantation. Recipient fetal liver and cord blood were analyzed for donor cell frequency and donor cell phenotype by dualcolor Row cytometry, Pertinent findings included the following: (1) a triphasic kinetic profile exists after in utero hematopoietic stem cell (HSC) transplantation (homing of circulating donor cells, rapid reduction of donorcell frequency, and donor cell competitive equilibration); (2) homing to the fetal liver is nonselective and reflects the phenotypic profile of the donor population; and (3) the kinetics after the prenatal transplantation ofcongenic or fully allogeneic cells are identical. This model will facilitate a systematic analysis of the mechanisms that regulate the homing of prenatally transplanted hematopoietic cells. (C) 1999 by The American Society of Hematology.

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Documento generato il 25/11/20 alle ore 10:14:27