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Titolo:
Creatine accumulation and exchange by HEK293 cells stably expressing high levels of a creatine transporter
Autore:
Dodd, JR; Zheng, T; Christie, DL;
Indirizzi:
Univ Auckland, Sch Biol Sci, Biochem & Mol Biol Grp, Auckland 1, New Zealand Univ Auckland Auckland New Zealand 1 l Biol Grp, Auckland 1, New Zealand
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
fascicolo: 1-2, volume: 1472, anno: 1999,
pagine: 128 - 136
SICI:
0304-4165(19991018)1472:1-2<128:CAAEBH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; RAT-BRAIN; FUNCTIONAL EXPRESSION; CULTURED-CELLS; CLONING; MUSCLE; EFFLUX; TISSUES; KINASE;
Keywords:
creatine transporter; creatine; accumulation; exchange;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Christie, DL Univ Auckland, Sch Biol Sci, Biochem & Mol Biol Grp, Private Bag 92019, Auckland 1, New Zealand Univ Auckland Private Bag 92019 Auckland New Zealand 1 aland
Citazione:
J.R. Dodd et al., "Creatine accumulation and exchange by HEK293 cells stably expressing high levels of a creatine transporter", BBA-GEN SUB, 1472(1-2), 1999, pp. 128-136

Abstract

We have generated a stable HEK293 cell line expressing high levels of a creatine transporter (CREAT). This cell line (HEK293-CREAT) was used to studythe properties of GREAT in terms of the accumulation and release of creatine. HEK293-CREAT cells accumulated high steady state levels of creatine under saturating creatine levels (approx. 25-fold higher intracellular creatine levels than seen in control cells). The accumulation of high levels of creatine affected [H-3]creatine uptake by decreasing the V-max for transport. High intracellular creatine levels were maintained in the absence of extracellular creatine. External creatine stimulated the release of stored creatine by an exchange mechanism dependent on extracellular Na+. These studies have shown that cellular creatine levels can be affected by the amount of creatine transporter in the membrane and exchange through the creatine transporter. These findings highlight the importance of the creatine transporterin the maintenance of intracellular creatine levels. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:48:22