Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Molecular cloning of macrophin, a human homologue of Drosophila kakapo with a close structural similarity to plectin and dystrophin
Autore:
Okuda, T; Matsuda, S; Nakatsugawa, S; Ichigotani, Y; Iwahashi, N; Takahashi, M; Ishigaki, T; Hamaguchi, M;
Indirizzi:
Nagoya Univ, Sch Med, Dept Mol Pathogenesis, Showa Ku, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 a Ku, Nagoya, Aichi 4668550, Japan Nagoya Univ, Sch Med, Dept Radiol, Showa Ku, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 a Ku, Nagoya, Aichi 4668550, Japan Nagoya Univ, Sch Med, Dept Pathol 2, Showa Ku, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 a Ku, Nagoya, Aichi 4668550, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 264, anno: 1999,
pagine: 568 - 574
SICI:
0006-291X(19991022)264:2<568:MCOMAH>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCULAR-DYSTROPHY; COILED-COIL; EPIDERMOLYSIS-BULLOSA; BETA-DYSTROBREVIN; CDNA CLONING; PROTEIN; MEMBER; FAMILY; GENE; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Hamaguchi, M Nagoya Univ, Sch Med, Dept Mol Pathogenesis, Showa Ku, 65 Turumai Cho, Nagoya, Aichi 4668550, Japan Nagoya Univ 65 Turumai Cho Nagoya Aichi Japan 4668550 , Japan
Citazione:
T. Okuda et al., "Molecular cloning of macrophin, a human homologue of Drosophila kakapo with a close structural similarity to plectin and dystrophin", BIOC BIOP R, 264(2), 1999, pp. 568-574

Abstract

We have determined the complete cDNA coding sequence of a novel cytoskeletal protein by the degenerative primer-mediated PCR strategy. This novel gene, named as macrophin (microfilament and actin filament cross-linker protein related to plectin and dystrophin, Accession No. AB029290), appears to bea human homologue of a Drosophila gene, kakapo and shows close similarity to plectin and dystrophin on the search of BLAST homology-computed database. Comparison of the deduced protein sequences for macrophin and kakapo revealed that they were 66% similar, and both of them have an NH2-terminal actin-binding domain, a central rod region composed of spectrin-like repeats, and COOH-terminal Gas2-related region, The predicted sequences for macrophinare 5430 amino acids in length with a calculated molecular mass of 620 kDa, which is one of the largest size idenfied in human cytoskeletal proteins. High expression of macrophin was observed in brain, heart, lung, placenta,liver, kidney, and pancreas. In pancreas, we found that macrophin was specifically expressed in acinar cells than islet cells by in situ hybridization. By using radiation hybrid panel, we have mapped the macrophin gene to the chromosome 1p31-32. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 00:45:39