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Titolo:
Osmoadaptation in halophilic and alkaliphilic methanotrophs
Autore:
Khmelenina, VN; Kalyuzhnaya, MG; Sakharovsky, VG; Suzina, NE; Trotsenko, YA; Gottschalk, G;
Indirizzi:
Univ Gottingen, Inst Mikrobiol & Genet, D-37077 Gottingen, Germany Univ Gottingen Gottingen Germany D-37077 net, D-37077 Gottingen, Germany Russian Acad Sci, Inst Biochem & Physiol Microorganisms, Pushchino 142292,Moscow Region, Russia Russian Acad Sci Pushchino Moscow Region Russia 142292 cow Region, Russia
Titolo Testata:
ARCHIVES OF MICROBIOLOGY
fascicolo: 5, volume: 172, anno: 1999,
pagine: 321 - 329
SICI:
0302-8933(199911)172:5<321:OIHAAM>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARINE METHANOTROPHS; MODERATE HALOPHILE; SALT CONCENTRATION; VIBRIO-COSTICOLA; AMINO-ACID; BACTERIA; ECTOTHIORHODOSPIRA; SALINITY; UKRAINE; LAYER;
Keywords:
Methylobacter alcaliphilus 20Z; Methylobacter modestohalophilus 10S osmoprotectants; ectoine; sucrose; 5-oxoproline; phospholipids; osmoadaptation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Gottschalk, G Univ Gottingen, Inst Mikrobiol & Genet, Grisebachstr 8, D-37077 Gottingen,Germany Univ Gottingen Grisebachstr 8 Gottingen Germany D-37077 any
Citazione:
V.N. Khmelenina et al., "Osmoadaptation in halophilic and alkaliphilic methanotrophs", ARCH MICROB, 172(5), 1999, pp. 321-329

Abstract

By using H-1- and C-13-NMR spectroscopy, an accumulation of sucrose and two cyclic amino acids [ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid) and 5-oxoproline (pyrrolidone carboxylic acid)] was detected in the halotolerant methanotrophs Methylobacter alcaliphilus 20Z and Methylobacter modestohalophilus 10S. The organic solute pool was found to increase upon raising the NaCl concentration. In M. alcaliphilus 202, the intracellular level of the total solutes was shown to be sufficient to balance the osmotic pressure of the medium, whereas in M. modestohalophilus 10S their content was several times lower. Additionally, phosphatidylglycerol and phosphatidylcholine were predominant cell phospholipids in salt-adapted M. alcaliphilus 202. However, no phosphatidylcholine was found in M. modestohalophilus 10S, and the portion of phosphatidylglycerol increased while phosphatidylethanolamine decreased upon elevated external NaCl concentrations. Regularly arranged glycoprotein surface layers (S-layers) of hexagonal and linear (p2) symmetry were observed on the outer cell walls of M. alcaliphilus 202 and M. modestohalophilus 10S. The S-layer in M alcaliphilus 202 consisting of tightly packed, cup-shaped subunits was lost during growth at pH 7.2 (the lowest possible pH) in the absence of NaCl. Hence, osmoadaptation in the methanotrophs studied involves structure/function alterations of cell envelopes and changes in the chemical composition of membranes as well as de novo synthesis of compatible solutes.

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Documento generato il 22/09/20 alle ore 11:30:55