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Titolo:
Entomologic and avian investigations of an epidemic of West Nile fever in Romania in 1996, with serologic and molecular characterization of a virus isolate from mosquitoes
Autore:
Savage, HM; Ceianu, C; Nicolescu, G; Karabatsos, N; Lanciotti, R; Vladimirescu, A; Laiv, L; Ungureanu, A; Romanca, C; Tsai, TF;
Indirizzi:
Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, Ft Collins, CO 80522 USA Ctr Dis Control & Prevent Ft Collins CO USA 80522 t Collins, CO 80522 USA Cantacuzino Inst, Dept Entomol, Bucharest 70100, Romania Cantacuzino InstBucharest Romania 70100 tomol, Bucharest 70100, Romania Romanian Ornithol Ctr, Bucharest 71592, Romania Romanian Ornithol Ctr Bucharest Romania 71592 , Bucharest 71592, Romania Army Ctr Med Res, Bucharest, Romania Army Ctr Med Res Bucharest RomaniaArmy Ctr Med Res, Bucharest, Romania Bucharest Prevent Med Ctr, Bucharest, Romania Bucharest Prevent Med Ctr Bucharest Romania Med Ctr, Bucharest, Romania
Titolo Testata:
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
fascicolo: 4, volume: 61, anno: 1999,
pagine: 600 - 611
SICI:
0002-9637(199910)61:4<600:EAAIOA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
JAPANESE ENCEPHALITIS; NUCLEOTIDE-SEQUENCE; MAXIMUM-LIKELIHOOD; DNA-SEQUENCES; PROTEINS; FLAVIVIRUS; PHYLOGENY; GENOME; TREES; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Savage, HM Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, POB 2087, Ft Collins, CO 80522 USA Ctr Dis Control & Prevent POB 2087 Ft Collins CO USA 80522 USA
Citazione:
H.M. Savage et al., "Entomologic and avian investigations of an epidemic of West Nile fever in Romania in 1996, with serologic and molecular characterization of a virus isolate from mosquitoes", AM J TROP M, 61(4), 1999, pp. 600-611

Abstract

Between July and October 1996, a West Nile (WN) fever epidemic occurred inthe southern plain and Danube Valley of Romania and in the capital city ofBucharest, resulting in hundreds of neurologic cases and 17 fatalities. Inearly October 1996, entomologic and avian investigations of the epidemic were conducted in the city of Bucharest and nearby rural areas. Thirty (41%)of 73 domestic fowl sampled had neutralizing antibody to WN virus, including 5 of 13 ducks (38%), 1 of I goose, 19 of 52 chickens (37%), 1 of 1 peahen, and 4 of 6 turkeys (67%). Seroprevalence in domestic fowl (27%, or 7 of 26) from the urban Bucharest site was not significantly different (P = 0.08, by Fisher's exact test) than rates at three rural sites (50%, or 23 of 46). Serum collected from one of 12 Passeriformes, an Erithacus rubecula, waspositive for neutralizing antibody to WN virus. A total of 5,577 mosquitoes representing seven taxa were collected. Culex pipiens pipiens accounted for 96% of the mosquitoes collected. A single virus isolate, RO97-50, was obtained from a pool of 30 Cx. p. pipiens females aspirated from the walls and ceiling of a blockhouse located near the center of Bucharest, resulting in a minimum infection rate of 0.19 per 1,000. Antisera prepared against RO97-50 failed to distinguish among RO97-50, WN virus strain Eg101, and Kunjin(KUN) virus strain MRM16. A 2,323-basepair DNA fragment of the envelope (E) glycoprotein gene from RO97-50 and a Romanian WN virus strain obtained from a human cerebrospinal fluid sample, RO96-1030, were sequenced. Phylogenetic analyses of 23 WN virus strains and one KUN virus strain using the amino acid and nucleotide sequences for a small portion of the E gene suggest the existence of two large lineages of viruses. Bootstrap analysis of the nucleotide alignment-indicated strong support (95%) for a lineage composed ofWN virus strains from northern Africa, including isolates from Egypt and Algeria, and west, central, and east Africa, all of the European isolates, those from France and Romania, an Israeli isolate, and an isolate of KUN virus from Australia. The nucleotide sequence of RO97-50 was identical to the sequence of a WN virus isolate obtained from Cx. neavei mosquitoes from Senegal and Cx. univittatus mosquitoes from Kenya. The phylogenetic analyses were compatible with the introduction of virus into Romania by birds migrating from sub-Saharan Africa, to northern Africa, and into southern Europe.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 20:55:43