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Titolo:
Absence of mitochondrial dysfunction in polymyalgia rheumatica - Evidence based on a simultaneous molecular and biochemical approach
Autore:
Miro, O; Jarreta, D; Casademont, J; Barrientos, A; Rodriguez, B; Gomez, M; Nunes, V; Urbano-Marquez, A; Cardellach, F;
Indirizzi:
Univ Barcelona, Dept Internal Med,Hosp Clin, Muscle Res Unit, August Pi I Sunyer Biomed Res Inst,Med Sch, SP-08036 Barcelona, Spain Univ Barcelona Barcelona Spain SP-08036 d Sch, SP-08036 Barcelona, Spain Oncol Res Inst, Dept Mol Genet, Barcelona, Spain Oncol Res Inst Barcelona Spain s Inst, Dept Mol Genet, Barcelona, Spain
Titolo Testata:
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY
fascicolo: 5, volume: 28, anno: 1999,
pagine: 319 - 323
SICI:
0300-9742(1999)28:5<319:AOMDIP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SKELETAL-MUSCLE; MYOPATHY; ARTHRITIS; DELETIONS; DNA;
Keywords:
polymyalgia rheumatica; mitochondria; mitochondrial DNA; electron transport chain; enzyme activity; oxidative activity; mitochondrial DNA deletions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Cardellach, F Univ Barcelona, Dept Internal Med,Hosp Clin, Muscle Res Unit, August Pi I Sunyer Biomed Res Inst,Med Sch, Villarroel 170, SP-08036 Barcelona, Spain Univ Barcelona Villarroel 170 Barcelona Spain SP-08036 pain
Citazione:
O. Miro et al., "Absence of mitochondrial dysfunction in polymyalgia rheumatica - Evidence based on a simultaneous molecular and biochemical approach", SC J RHEUM, 28(5), 1999, pp. 319-323

Abstract

Objective: To investigate the molecular and biochemical profile of skeletal muscle mitochondria of patients with isolated polymyalgia rheumatica (PMR). Patients and Methods: We included patients with a recent diagnosis of PMR and as control healthy individuals submitted to orthopedic surgery. Skeletal muscle was obtained from quadriceps, thus was mitochondria immediately isolated. Long polymerase chain reaction and Southern blot transference were performed to detect deleted mtDNA molecules. Mitochondrial oxidative activity using different substrates and individual enzyme activity of respiratorychain complexes were assessed to search for any biochemical dysfunction. Results: Fifty-one individuals (PMR=25, controls=26) were included. Mean age was 72 (11) years; 45% were females. We found no significant increase ofdeleted mtDNA molecules in PMR patients compared to controls. Both groups differed neither on oxygen consumption (p=NS for all substrates) nor enzymatic activity (p=NS for all complexes). Conclusions: Skeletal muscle mitochondria are molecularly and biochemically unaffected in PMR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:52:37