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Titolo:
Formation of a carcinogenic aromatic amine from an azo dye by human skin bacteria in vitro
Autore:
Platzek, T; Lang, C; Grohmann, G; Gi, US; Baltes, W;
Indirizzi:
Fed Inst Hlth Protect Consumers & Vet Med, D-14191 Berlin, Germany Fed Inst Hlth Protect Consumers & Vet Med Berlin Germany D-14191 Germany Tech Univ Berlin, Inst Microbiol & Genet, D-13355 Berlin, Germany Tech Univ Berlin Berlin Germany D-13355 & Genet, D-13355 Berlin, Germany Tech Univ Berlin, Inst Food Chem, D-13355 Berlin, Germany Tech Univ Berlin Berlin Germany D-13355 od Chem, D-13355 Berlin, Germany
Titolo Testata:
HUMAN & EXPERIMENTAL TOXICOLOGY
fascicolo: 9, volume: 18, anno: 1999,
pagine: 552 - 559
SICI:
0960-3271(199909)18:9<552:FOACAA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN INTESTINAL MICROFLORA; ACTIVATION; REDUCTION; MUTAGENICITY; BENZIDINE; ASSAY;
Keywords:
azo dyes; skin bacteria; metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Platzek, T Fed Inst Hlth Protect Consumers & Vet Med, Postfach 330013, D-14191 Berlin, Germany Fed Inst Hlth Protect Consumers & Vet Med Postfach 330013 Berlin Germany D-14191
Citazione:
T. Platzek et al., "Formation of a carcinogenic aromatic amine from an azo dye by human skin bacteria in vitro", HUM EXP TOX, 18(9), 1999, pp. 552-559

Abstract

Azo dyes represent the major class of dyestuffs. They are metabolised to the corresponding amines by liver enzymes and the intestinal microflora following incorporation by both experimental animals and humans. For safety evaluation of the dermal exposure of consumers to azo dyes from wearing coloured textiles, a possible cleavage of azo dyes by the skin microflora should be considered since, in contrast to many dyes, aromatic amines are easily absorbed by the skin. A method for measuring the ability of human skin nora to reduce azo dyes was established. In a standard experiment, 3 x 10(11) cells of a culture of Staphylococcus aureus were incubated in synthetic sweat(pH 6.8, final volume 20 mL) at 28 degrees C for 24 h with Direct Blue 14 (C.I. 23850, DE 14). The reaction products were extracted and analysed using HPLC. The reduction product o-tolidine (3,3'-dimethylbenzidine, OT) couldindeed be detected showing that the strain used was able to metabolise DE 14 to the corresponding aromatic amine, In addition to OT, two further metabolites of DB 14 were detected. Using mass spectrometry they were identified as 3,3'-dimethyl-4-amino-4'-hydroxybiphenyl and 3,3'-dimethyl-4-aminobiphenyl, The ability to cleave azo dyes seems to be widely distributed among human skin bacteria, as, under these in vitro conditions, bacteria isolated from healthy human skin and human skin bacteria from strain collections also exhibited azo reductase activity. Further studies are in progress in order to include additional azo dyes and coloured textiles. At the moment, the meaning of the results with regard to consumer health cannot be finally assessed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 09:35:46