Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The effects of GM-CSF, steel factor and MIP-1 alpha on the expression and activation of Cdc25A phosphatase in Mo7e cells
Autore:
Reid, S; Broxmeyer, HE;
Indirizzi:
Indiana Univ, Sch Med, Walther Oncol Ctr, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ol Ctr, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Microbiol Immunol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 mmunol, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Med, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 pt Med, Indianapolis, IN 46202 USA Walther Canc Inst, Indianapolis, IN USA Walther Canc Inst Indianapolis INUSA er Canc Inst, Indianapolis, IN USA
Titolo Testata:
CYTOKINES CELLULAR & MOLECULAR THERAPY
fascicolo: 3, volume: 5, anno: 1999,
pagine: 129 - 138
SICI:
1368-4736(199909)5:3<129:TEOGSF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; CYCLIN-DEPENDENT KINASES; DNA-DAMAGE CHECKPOINT; C-KIT LIGAND; MITOTIC INDUCER; TYROSINE PHOSPHATASE; G(1)/S TRANSITION; PROTEIN-KINASE; FISSION YEAST; BONE-MARROW;
Keywords:
cell proliferation; Mo7e; GM-CSF; steel factor; MIP-1 alpha;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Broxmeyer, HE Indiana Univ, Sch Med, Walther Oncol Ctr, 1044 W Walnut St, Indianapolis, IN 46202 USA Indiana Univ 1044 W Walnut St Indianapolis IN USA 46202 USA
Citazione:
S. Reid e H.E. Broxmeyer, "The effects of GM-CSF, steel factor and MIP-1 alpha on the expression and activation of Cdc25A phosphatase in Mo7e cells", CYTOK CELL, 5(3), 1999, pp. 129-138

Abstract

Active cyclin-dependent kinases (CDKs) are required for progression through the G(1) phase of the cell cycle and entry into S phase. Activity of G(1)CDKs is controlled by mechanisms including phosphorylation of Thr14 and Tyr15 residues. Removal of inhibitory phosphates on these amino acid residuesis required for G(1) CDK activation, and is mediated by the Cdc25A phosphatase. Regulation of active Cdc25A phosphatase levels may be important for the proliferation of hematopoietic progenitor cells, effects assessed in thehuman growth-factor-dependent cell line Mo7e. Constitutive Cdc25A protein levels were enhanced with granulocyte-macrophage colony-stimulating factor (GM-CSF) plus steel factor (SF). Cdc25A is thought to exert its activity inthe nucleus, and nuclear protein levels of Cdc25A were also enhanced with GM-CSF and SF. GMCSF plus SF promote synergistic growth of Mo7e cells. Pretreatment with macrophage inflammatory protein (MIP-1 alpha) inhibited GM-CSF- plus SF-induced growth and upregulation of Cdc25A protein levels. Stimulation with GM-CSF and SF also rapidly increased Cdc25A phosphatase activity, an effect suppressed by MIP-1 alpha. A concomitant inhibition of increased CDK4 kinase activity correlated with increased phosphotyrosine levels on CDK4 when cells were pretreated with MIP-1 alpha prior to GM-CSF and SF. These data suggest that Cdc25A expression and activity are regulated during proliferation of Mo7e cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:03:44