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Titolo:
REVERSE-TRANSCRIPTASE AND CORRESPONDING ACTIVITY-BLOCKING ANTIBODY FOR MONITORING SIVSM INFECTION IN MACAQUES
Autore:
EKSTRAND DHL; BOTTIGER D; ANDERSSON H; GRONOWITZ JS; CLAS FRK;
Indirizzi:
CAVIDITECH AB,UPPSALA SCI PK S-75183 UPPSALA SWEDEN CAVIDITECH AB S-75183 UPPSALA SWEDEN UNIV UPPSALA,BMC,DEPT MED GENET S-75123 UPPSALA SWEDEN SMI,MEDIVIR AB S-10521 STOCKHOLM SWEDEN UPPSALA UNIV,BMC,DEPT MOL BIOL S-75124 UPPSALA SWEDEN
Titolo Testata:
AIDS research and human retroviruses
fascicolo: 7, volume: 13, anno: 1997,
pagine: 601 - 610
SICI:
0889-2229(1997)13:7<601:RACAAF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; MONKEYS MACACA-FASCICULARIS; NON-B HEPATITIS; HTLV-III LAV; MONOCLONAL-ANTIBODIES; INHIBITING ANTIBODY; DISEASE PROGRESSION; NON-A; SERUM; HIV-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
D.H.L. Ekstrand et al., "REVERSE-TRANSCRIPTASE AND CORRESPONDING ACTIVITY-BLOCKING ANTIBODY FOR MONITORING SIVSM INFECTION IN MACAQUES", AIDS research and human retroviruses, 13(7), 1997, pp. 601-610

Abstract

A nonradioactive reverse transcriptase (RT) assay was used to measureRT activity in serum during the viremia peak associated with primary infection and for measuring the generation and maintenance of RT activity-blocking antibody (RTb-ab) titers during and after seroconversion in SIV-infected macaques, The RT assay was compared to an antigen capture immunoassay designed for HIV-2/SIVsm and was found to be approximately 40 times more sensitive in detecting SIVsm in serum from infectedmacaques, The RT assay detected RT activity in serum corresponding tolevels from 3 pg/ml. Earliest detection of viral replication using the RT assay was on day 6-8, with a peak at day 10 (up to 8000 pg/ml), The earliest detection of RTb-ab was seen on day 17-23, with established RTb-ab titers by day 29, followed by increasing titers of 15,000-120,000 by day 62-77, The usefulness of RT and RTb-ab for monitoring the course of SIV infection in monkey models is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 16:14:12