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Titolo:
THE MULTIPLE INHIBITORY MECHANISMS OF GEM 91(R), A GAG ANTISENSE PHOSPHOROTHIOATE OLIGONUCLEOTIDE, FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1
Autore:
YAMAGUCHI K; PAPP B; ZHANG DZ; ALI AN; AGRAWAL S; BYRN RA;
Indirizzi:
HARVARD UNIV,SCH MED,BETH ISRAEL DEACONESS MED CTR,DIV EXPT MED,1 DEACONESS RD BOSTON MA 02215 HARVARD UNIV,SCH MED,BETH ISRAEL DEACONESS MED CTR,DIV EXPT MED BOSTON MA 02215 HARVARD UNIV,SCH MED,BETH ISRAEL DEACONESS MED CTR,DIV HEMATOL ONCOL BOSTON MA 02215 HYBRIDON INC CAMBRIDGE MA 02139
Titolo Testata:
AIDS research and human retroviruses
fascicolo: 7, volume: 13, anno: 1997,
pagine: 545 - 554
SICI:
0889-2229(1997)13:7<545:TMIMOG>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE-SPECIFIC INHIBITION; CHRONICALLY INFECTED-CELLS; GENE-EXPRESSION; REVERSE-TRANSCRIPTASE; SECONDARY STRUCTURE; COLORIMETRIC ASSAY; THERAPEUTIC AGENT; HIV; OLIGODEOXYNUCLEOTIDES; REPLICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
K. Yamaguchi et al., "THE MULTIPLE INHIBITORY MECHANISMS OF GEM 91(R), A GAG ANTISENSE PHOSPHOROTHIOATE OLIGONUCLEOTIDE, FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1", AIDS research and human retroviruses, 13(7), 1997, pp. 545-554

Abstract

GEM 91 (gene expression modulator) is a 25-mer oligonucleotide phosphorothioate complementary to the gag initiation site of HIV-1. GEM 91 has been studied in various in vitro cell culture models to examine inhibitory effects on different stages of HIV-1 replication. Experiments were focused on the binding of virions to the cell surface, inhibitionof virus entry, reverse transcription (HIV DNA production), inhibition of steady state viral mRNA levels, inhibition of virus production from chronically infected cells, and inhibition of HIV genome packaging within virions. Experiments were also performed in vitro in an attemptto generate strains of HIV with reduced sensitivity to GEM 91, We observed sequence-dependent inhibition of virus entry/reverse transcription and a reduction in steady state viral RNA levels, We also observed sequence-independent inhibition of virion binding to cells and inhibition of virus production by chronically infected cells, Using in vitro methods that were successful in generating HIV strains with reduced sensitivity to AZT, we were unable to generate strains with reduced sensitivity to GEM 91.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 05:03:04