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Titolo:
Frontiers in tissue engineering - In vitro modulation of chondrogenesis
Autore:
Freed, LE; Martin, I; Vunjak-Novakovic, G;
Indirizzi:
MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA MIT Cambridge MA USA02139 iv Hlth Sci & Technol, Cambridge, MA 02139 USA
Titolo Testata:
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
fascicolo: 367, , anno: 1999, supplemento:, S
pagine: S46 - S58
SICI:
0009-921X(199910):367<S46:FITE-I>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE REPAIR; BIODEGRADABLE POLYMER SCAFFOLDS; MAMMALIAN CHONDROCYTES INVITRO; MARROW STROMAL CELLS; BONE-MARROW; ALGINATE BEADS; COLLAGEN; CULTURE; MATRIX; PHENOTYPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Freed, LE MIT, Div Hlth Sci & Technol, E25-342,45 Carleton St, Cambridge, MA 02139 USA MIT E25-342,45 Carleton St Cambridge MA USA 02139 , MA 02139 USA
Citazione:
L.E. Freed et al., "Frontiers in tissue engineering - In vitro modulation of chondrogenesis", CLIN ORTHOP, (367), 1999, pp. S46-S58

Abstract

Cartilage tissue engineering can provide functional cartilaginous constructs that can be used for controlled in vitro studies of chondrogenesis and potentially for in vivo articular cartilage repair. Ideally, engineered cartilage should be indistinguishable from native articular cartilage with respect to zonal organization, biochemical composition, and mechanical properties. In the model system presented here, chondrogenic cells are expanded in vitro as required, seeded onto three-dimensional polymeric scaffolds, and cultured in bioreactor vessels. During the course of in vitro cultivation, construct cellularity plateaus at a physiologic level, fractions of glycosaminoglycan and Type II collagen increase progressively, and the scaffold biodegrades. Construct structure (composition, morphology) and function (biosynthetic activity, mechanical properties) depend on cultivation conditions. This paper reviews recent studies of in vitro modulation of chondrogenesis by: (1) cell seeding density and source: (2) the tissue regeneration template; (3) biochemical regulatory signals; (4) mixing, mass transport and hydrodynamic forces; and (5) cultivation time. Key requirements and some of thecritical research needs for successful cartilage tissue engineering are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:58:15