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Titolo:
Ultrastructural alterations during the critical phase of reperfusion: A stereological study in buffer-perfused isolated rat hearts
Autore:
Hegstad, AC; Ytrehus, K; Lindal, S; Jorgensen, L;
Indirizzi:
Univ Tromso, Inst Med Biol, Dept Morphol, N-9037 Tromso, Norway Univ Tromso Tromso Norway N-9037 ol, Dept Morphol, N-9037 Tromso, Norway Univ Tromso, Inst Med Biol, Dept Med Physiol, N-9037 Tromso, Norway Univ Tromso Tromso Norway N-9037 Dept Med Physiol, N-9037 Tromso, Norway
Titolo Testata:
CARDIOVASCULAR PATHOLOGY
fascicolo: 5, volume: 8, anno: 1999,
pagine: 279 - 289
SICI:
1054-8807(199909/10)8:5<279:UADTCP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
IRREVERSIBLE MYOCARDIAL INJURY; CORONARY-ARTERY OCCLUSION; OXYGEN RADICALS; MYOCYTIC MITOCHONDRIA; ISCHEMIC MYOCARDIUM; MEMBRANE DAMAGE; CELL INJURY; PEROXIDATION; MECHANISM; THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Hegstad, AC Univ Tromso, Inst Med Biol, Dept Morphol, N-9037 Tromso, Norway Univ Tromso Tromso Norway N-9037 phol, N-9037 Tromso, Norway
Citazione:
A.C. Hegstad et al., "Ultrastructural alterations during the critical phase of reperfusion: A stereological study in buffer-perfused isolated rat hearts", CARDIO PATH, 8(5), 1999, pp. 279-289

Abstract

The present study focuses on myocardial ultrastructural alterations duringthe early phase of reperfusion. Isolated buffer-perfused rat hearts were exposed to standard perfusion (control group, n = 10); 60 min of global ischemia (n = 10); 60 min of global ischemia followed by 2 min of reperfusion (n = 10); or 60 min of global ischemia followed by 10 min of reperfusion (n = 10). The hearts were perfusion-fixed for electron microscopy, and ultrastructural evaluation was performed using stereological technique in order toobtain an estimate of the volume fraction and absolute volume of differenttissue components. Effect of ischemia: Neither the ventricular nor the myocytic volume differed significantly from the respective control values. Both the myocytic mitochondrial volume (135 +/- 8 vs control 89 +/- mu l) and the volume of myocytic clear space (35 +/- 6 vs control 10 +/- 2 mu l) weresignificantly increased. The capillary volume (22 +/- 4 vs control 58 +/- 6 mu l) and the volume of the capillary lumen (15 +/- 3 vs control 48 +/- 5mu l) were significantly decreased. The volume of the capillary wall, however, was not altered after exposure to ischemia (7 +/- 3 vs control 10 +/- 1 mu l) Additive effect of ischemia and reperfusion: Both the ventricular volume (755 +/- 28 vs control 600 +/- 32 mu l) and the myocytic volume (396 /- 24 vs control 287 +/- 16 mu l) were significantly increased after 10 min of reperfusion. Effect of reperfusion: The ischemic-induced myocytic mitochondrial swelling and increase of clear space were not reinforced during reperfusion. Furthermore, the volume of the capillary lumen and the capillary wall did not alter significantly in the groups exposed to reperfusion compared to the ischemic hearts. In conclusion, stereological evaluation did not reveal significant aggravation of ischemic-induced myocardial injury during the early phase of reperfusion. (C) 1999 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:11:56