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Titolo:
Distribution and regulation of cyclooxygenase-2 in carrageenan-induced inflammation
Autore:
Nantel, F; Denis, D; Gordon, R; Northey, A; Cirino, M; Metters, KM; Chan, CC;
Indirizzi:
Merck Frosst Ctr Therapeut Res, Dept Biochem & Mol Biol, Pointe Claire, PQH9R 4P8, Canada Merck Frosst Ctr Therapeut Res Pointe Claire PQ Canada H9R4P8 P8, Canada Merck Frosst Ctr Therapeut Res, Dept Pharmacol, Pointe Claire, PQ H9R 4P8,Canada Merck Frosst Ctr Therapeut Res Pointe Claire PQ Canada H9R 4P8 4P8,Canada
Titolo Testata:
BRITISH JOURNAL OF PHARMACOLOGY
fascicolo: 4, volume: 128, anno: 1999,
pagine: 853 - 859
SICI:
0007-1188(199910)128:4<853:DAROCI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTAGLANDIN G/H SYNTHASE-1; MESSENGER-RNA; EXPRESSION; RAT; COX-2; MACROPHAGES; INHIBITORS; CLONING; DRUGS; CELLS;
Keywords:
prostaglandin; cyclooxygenase; inflammation; antibodies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Nantel, F Merck Frosst Ctr Therapeut Res, Dept Biochem & Mol Biol, POB 1005, Pointe Claire, PQ H9R 4P8, Canada Merck Frosst Ctr Therapeut Res POB 1005 Pointe Claire PQ Canada H9R 4P8
Citazione:
F. Nantel et al., "Distribution and regulation of cyclooxygenase-2 in carrageenan-induced inflammation", BR J PHARM, 128(4), 1999, pp. 853-859

Abstract

1 We characterized the regulation of cyclooxygenase-2 (COX-2) at the mRNA,protein and mediator level in two rat models of acute inflammation, carrageenan-induced paw oedema and mechanical hyperalgesia.2 Carrageenan was injected in the hind paw of rat at low (paw oedema) and high doses (hyperalgesia). COX-2 and prostaglandin E-2 (PGE(2)) levels weremeasured by RT-PCR and immunological assays. We also determined the distribution of COX-2 by immunohistochemistry.3 The injection of carrageenan produced a significant and parallel induction of both COX-2 and PGE(2). This induction was significantly higher in hyperalgesia than in paw oedema. This was probably due to the 9 fold higher concentration of carrageenan used to provoke hyperalgesia.4 Immunohistochemical examination showed COX-2 immunoreactivity in the epidermis, skeletal muscle and inflammatory cells of rats experiencing hyperalgesia. In paw oedema however, only the epidermis showed positive COX-2 immunoreactivity.5 Pretreatment with indomethacin completely abolished the induction of COX-2 in paw oedema but not in hyperalgesia.6 These results suggest that multiple mechanisms regulate COX-2 induction especially in the more severe model. In carrageenan-induced paw oedema, prostanoid production have been linked through the expression of the COX-2 gene which suggest the presence of a positive feedback loop mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:19:26