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Titolo:
Inactivation of microsomal triglyceride transfer protein impairs the normal redistribution but not the turnover of newly synthesized glycerolipid in the cytosol, endoplasmic reticulum and Golgi of primary rat hepatocytes
Autore:
Hebbachi, AM; Gibbons, GF;
Indirizzi:
Univ Oxford, Radcliffe Infirm, Nuffield Dept Clin Med, Metab Res Lab,Oxford Lipid Metab Grp, Oxford OX2 6HE, England Univ Oxford Oxford England OX26HE id Metab Grp, Oxford OX2 6HE, England
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 1, volume: 1441, anno: 1999,
pagine: 36 - 50
SICI:
1388-1981(19991018)1441:1<36:IOMTTP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY LIPOPROTEIN; APO-B SECRETION; APOLIPOPROTEIN-B; HEPG2 CELLS; PHOSPHATIDYLCHOLINE BIOSYNTHESIS; PHOSPHOLIPID-COMPOSITION; CULTURED-HEPATOCYTES; MCA-RH7777 CELLS; OROTIC-ACID; FATTY-ACIDS;
Keywords:
very low-density lipoprotein; apolipoprotein B; triacylglycerol; phospholipid; subcellular; cell culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Gibbons, GF Univ Oxford, Radcliffe Infirm, Nuffield Dept Clin Med, Metab Res Lab,Oxford Lipid Metab Grp, Woodstock Rd, Oxford OX2 6HE, England Univ Oxford Woodstock Rd Oxford England OX2 6HE 6HE, England
Citazione:
A.M. Hebbachi e G.F. Gibbons, "Inactivation of microsomal triglyceride transfer protein impairs the normal redistribution but not the turnover of newly synthesized glycerolipid in the cytosol, endoplasmic reticulum and Golgi of primary rat hepatocytes", BBA-MOL C B, 1441(1), 1999, pp. 36-50

Abstract

The requirements for microsomal triglyceride transfer protein (MTP) duringthe turnover and transfer of glycerolipids from intracellular compartmentsinto secretory very low-density lipoprotein (VLDL) were studied by pre-labelling lipids with [H-3]glycerol and [C-14]oleate in primary cultures of rat hepatocytes. The intracellular redistribution of pre-labelled glycerolipids was then compared at the end of subsequent chase periods during which the MTP inhibitor BMS-200150 was either present or absent in the medium. Inhibition of MTP resulted in a decreased output of VLDL triacylglycerol (TAG) and a delayed removal of labelled TAG from the cytosol and from the membranes of the smooth endoplasmic reticulum (SER), the cis- and the trans-Golgi. Inactivation of MTP did not decrease the bulk lipolytic turnover of cellular TAG as reflected by changes in its [H-3]glycerol:[C-14]oleate ratios. However, a larger proportion of the resultant TAG fatty acids was re-esterified and remained with the membranes of the various subcellular fractions rather than emerging as VLDL. The effects of BMS-200150 on the pattern of phospholipid (PL) mechanism and redistribution suggested that inhibition of MTPprevented the normal lipolytic transfer of PL-derived fatty acids out of the SER, cis- and trans-Golgi membrane pools. Finally, changes in the C-14 specific radioactivities of the cytosolic and membrane pools of TAG suggested that inhibition of MTP prevented a normal influx of relatively unlabelledfatty acids into these pools during the chase period. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:55:08