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Titolo:
Cadmium uptake and transepithelial transport in control and long-term exposed Caco-2 cells: The role of metallothionein
Autore:
Blais, A; Lecoeur, S; Milhaud, G; Tome, D; Kolf-Clauw, M;
Indirizzi:
Ecole Natl Vet, Toxicol Lab, F-94704 Maisons Alfort, France Ecole Natl Vet Maisons Alfort France F-94704 4704 Maisons Alfort, France Inst Natl Agron, Lab Nutr Humaine & Physiol Intestinale, F-75231 Paris, France Inst Natl Agron Paris France F-75231 Intestinale, F-75231 Paris, France
Titolo Testata:
TOXICOLOGY AND APPLIED PHARMACOLOGY
fascicolo: 1, volume: 160, anno: 1999,
pagine: 76 - 85
SICI:
0041-008X(19991001)160:1<76:CUATTI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL EPITHELIAL-CELLS; GASTROINTESTINAL ABSORPTION; LLC-PK1 CELLS; MUCOSAL METALLOTHIONEIN; LINE CACO-2; IN-VIVO; RAT; MODEL; MICE; CD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Blais, A Ecole Natl Vet, Toxicol Lab, F-94704 Maisons Alfort, France EcoleNatl Vet Maisons Alfort France F-94704 ons Alfort, France
Citazione:
A. Blais et al., "Cadmium uptake and transepithelial transport in control and long-term exposed Caco-2 cells: The role of metallothionein", TOX APPL PH, 160(1), 1999, pp. 76-85

Abstract

Exposure of humans to cadmium, a common environmental pollutant, is mainlythrough food intake. However, the mechanisms of intestinal absorption havenot been clearly elucidated for this toxic metal ion. In order to investigate the effects of long-term exposure to this metal and the role of metallothioneins in cadmium absorption, we used human-derived Caco-2 cells cultured on porous membrane filters. We first validated this model by quantifying metal uptake and transepithelial transport on control cells and cells adapted to grow for 2 to 5 weeks in the presence of low doses of cadmium in the culture medium. The nontoxic doses of cadmium (0.1, 1.0, and 5 mu M), in which Caco-2 cells could be cultured for many passages without deleterious effects, were determined by evaluating transepithelial resistance of the cells and lactate dehydrogenase leakage. After 24 h of 1 mu M Cd exposure, intracellular cadmium levels were 3- and 6-fold higher for cells exposed for extended periods to 1 and 5 mu M cadmium, respectively, compared to control cells. In control and long-term exposed cells, this accumulation was inhibited by zinc, copper, and pCMBS, but not by verapamil or ouabain. Intracellular metallothionein content was increased 1.5-, 5-, and 12-fold for the cells grown in the presence of 0.1, 1.0, and 5 mu M cadmium, respectively, in the culture medium. The amount of metallothionein synthesized and released by the cells was highly correlated with cadmium accumulation and transport. Our results suggest that Caco-2 cell monolayers are a goad predictive modelfor the study of cadmium intestinal absorption following exposure to repeated low doses of cadmium, and confirm the essential role of metallothioneinin the regulation of cadmium intestinal absorption. (C) 1999 Academic Press.

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Documento generato il 13/07/20 alle ore 08:01:12